首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠酰基化饥饿素(AG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AG含量。大鼠酰基化饥饿素（AG）是一种由胃内分泌细胞产生的激素，具有促进食欲和调节能量平衡的作用。其活性形式是通过辛酰化修饰的，能够激活GHSR受体，影响生长激素释放、食欲和能量代谢。研究显示，AG在调节血糖和胰岛素抵抗中扮演重要角色，其水平变化与肥胖和糖尿病等代谢性疾病密切相关。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于动物模型研究，如肥胖、糖尿病或摄食调控机制的探索，也可用于体外药物筛选或激素分泌相关的基础研究，为科研人员提供稳定可靠的数据支持，满足代谢调控、内分泌系统研究等领域的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AG
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 87-101 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-98 | |||||
回收率：
| The recovery of rat AG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-104 | ||||
| EDTA plasma (n=4) | 97 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6902.6992.6952.5022001.8851.8991.8921.6991001.1621.1981.1800.987500.7980.7600.7790.586250.5810.5350.5580.36512.50.3880.3760.3820.1896.250.3010.3210.3110.11800.1910.1950.193 | ||||||
货期：3-5 working days
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