| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHROW21; CST6; CSTB; Cystatin B (stefin B); CYTB EPM1; EPM1A; PME; STFB; ULD;;Cystatin B |
| WB Predicted band size | Calculated MW: 11 kDa ; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Cystatin B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于Cystatin B抗体的参考文献及其摘要概括：
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1. **文献名称**: *Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1)*
**作者**: Pennacchio, L.A. et al. (1996)
**摘要**: 该研究首次克隆人类CSTB基因并发现其突变与EPM1疾病相关。文中使用Cystatin B特异性抗体验证基因表达水平，证明患者脑组织中Cystatin B蛋白表达显著降低，揭示了其在神经元功能中的关键作用。
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2. **文献名称**: *Cellular localization and functional analysis of the cystatin B protein in EPM1*
**作者**: Alakurtti, K. et al. (2005)
**摘要**: 通过免疫荧光和Western blot技术，利用Cystatin B抗体研究其亚细胞定位。结果显示Cystatin B不仅存在于溶酶体，还在线粒体和细胞核中有分布，提示其可能参与氧化应激调控和DNA修复。
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3. **文献名称**: *Cystatin B regulates innate immunity during HIV-1 infection*
**作者**: Stivahtis, G.L. et al. (2017)
**摘要**: 研究使用Cystatin B抗体探究其在HIV感染中的作用，发现其通过抑制溶酶体蛋白酶活性调控树突状细胞抗原呈递，影响抗病毒免疫反应，为HIV治疗提供新靶点。
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4. **文献名称**: *Antibody-based detection of cystatin B in neurodegenerative disorders*
**作者**: Parker, J.R. et al. (2015)
**摘要**: 开发高特异性Cystatin B抗体用于阿尔茨海默病和帕金森病患者脑脊液检测，发现其表达水平与神经炎症标志物正相关，提示其作为神经退行性疾病生物标志物的潜力。
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以上文献均涉及Cystatin B抗体的实验应用，涵盖疾病机制、亚细胞定位及临床诊断等领域。
Cystatin B, a member of the cystatin superfamily of cysteine protease inhibitors, is a small, ubiquitously expressed protein that regulates the activity of cathepsins and other cysteine proteases. It plays a critical role in maintaining protease-antiprotease balance, influencing cellular processes such as protein degradation, apoptosis, and inflammation. Primarily localized in the cytoplasm and nuclei, cystatin B is also implicated in lysosomal function and neuronal protection. Mutations in the CSTB gene encoding cystatin B are linked to progressive myoclonus epilepsy type 1 (EPM1), a rare neurodegenerative disorder, highlighting its importance in neurological health.
Antibodies targeting cystatin B are essential tools for studying its expression, localization, and function. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into disease mechanisms. In EPM1 research, cystatin B antibodies help characterize protein loss or dysfunction caused by CSTB mutations. Beyond neurology, they are used in cancer studies, as altered cystatin B levels correlate with tumor progression and metastasis. Additionally, these antibodies contribute to understanding immune responses, as cystatin B modulates inflammatory pathways.
Commercial cystatin B antibodies are typically validated for specificity and sensitivity across species, with applications in both basic and clinical research. Their development supports biomarker discovery and therapeutic targeting in diseases involving proteolytic imbalance.
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