首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CD68分子，CD68酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD68含量。CD68是一种在单核细胞、巨噬细胞等免疫细胞上高度表达的跨膜糖蛋白。它参与细胞内吞、信号传导等多种生理过程。当前研究聚焦其在炎症、肿瘤等疾病中的作用机制，有望为相关疾病的诊断和治疗提供新靶点和思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于多种科研场景，包括巨噬细胞活化状态分析、炎症性疾病动物模型研究（如关节炎或动脉粥样硬化）、组织修复过程中巨噬细胞浸润评估等实验需求。支持科研人员高效开展免疫细胞功能研究、病理机制探索及药物干预效果评价等实验，尤其适用于心血管、肿瘤微环境及免疫代谢相关领域的样本检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CD68
Uniprot No.：Q4FZY1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-98 | |||||
回收率：
| The recovery of rat CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 101 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.2102.1882.1992.063201.4841.4441.4641.328100.9711.0080.9900.85450.6220.6450.6340.4982.50.3780.3870.3830.2471.250.3040.2990.3020.1660.6250.2220.2120.2170.08100.1380.1340.136 | ||||||
货期：3-5 working days
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