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产品描述：人心钠肽(ANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液样本中的NPPA含量。NPPA（心房钠尿肽A）是一种利钠肽家族的蛋白质，参与调节细胞外液量和电解质稳态。其研究机制涉及利钠肽在心血管系统中的作用，包括调节血压、利尿和钠排泄。NPPA基因突变与多种疾病相关，如高血压和房颤。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可利用本产品开展心血管疾病动物模型、细胞水平机制研究，或用于药物研发中体外评估化合物对ANP分泌的影响。其操作便捷，与多种生物样本兼容，适合实验室常规检测及高通量筛选需求，为心血管生物学及代谢调控领域提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANF ELISA Kit; ANF_HUMAN ELISA Kit; ANP ELISA Kit; ATFB6 ELISA Kit; Atrial natriuretic factor ELISA Kit; Atrial natriuretic peptide ELISA Kit; Atriopeptin ELISA Kit; Cardiodilatin ELISA Kit; Cardiodilatin related peptide ELISA Kit; Cardiodilatin-related peptide ELISA Kit; Cardionatrin ELISA Kit; CDD ANF ELISA Kit; CDD ELISA Kit; CDD-ANF ELISA Kit; CDP ELISA Kit; Natriuretic peptide A ELISA Kit; Natriuretic peptide precursor A ELISA Kit; NPPA ELISA Kit; PND ELISA Kit; Prepronatriodilatin ELISA Kit
缩写：NPPA
Uniprot No.：P01160
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 95-108 | |||||
回收率：
| The recovery of human ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.9322.7892.8612.77110002.1782.1322.1552.0655001.5661.5551.5611.4712500.7890.7780.7840.6941250.4620.4560.4590.36962.50.2780.2670.2730.18331.250.2260.2180.2220.13200.0930.0870.090 | ||||||
货期：3-5 working days
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