| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDNB1; Ube4b; UBOX3; UFD2; UFD2a; Ufd2p;;UBE4B |
| WB Predicted band size | Calculated MW: 146 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human UBE4B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于UBE4B抗体的3篇代表性文献的简要概括：
1. **《UBE4B promotes axonal degeneration in Parkinson's disease models》**
*作者：Chen, L., et al. (2020)*
摘要：本研究利用特异性UBE4B抗体，发现UBE4B通过调控α-synuclein的泛素化水平，促进多巴胺能神经元轴突退化。抗体免疫荧光染色显示UBE4B在帕金森病模型中的异常聚集。
2. **《Antibody-based profiling of UBE4B in colorectal cancer progression》**
*作者：Wang, Y., et al. (2018)*
摘要：通过开发高特异性UBE4B单克隆抗体，研究揭示UBE4B在结直肠癌组织中高表达，且与肿瘤转移正相关。Western blot和免疫组化数据表明UBE4B可作为潜在预后标志物。
3. **《UBE4B interacts with CHMP4B to regulate endosomal trafficking》**
*作者：Suzuki, A., et al. (2016)*
摘要：使用UBE4B抗体进行Co-IP实验，发现UBE4B与ESCRT-III复合物成员CHMP4B相互作用，调控内吞体分选过程。研究揭示了UBE4B在膜运输中的新功能。
注：上述文献为示例，实际引用时建议通过PubMed或Google Scholar以“UBE4B antibody”为关键词检索最新文章，并核实作者及发表年份。
**Background of UBE4B Antibody**
UBE4B (Ubiquitination Factor E4B), also known as UFD2a, is a ubiquitin ligase enzyme critical in the ubiquitin-proteasome system (UPS), which regulates protein degradation and cellular homeostasis. It facilitates the attachment of ubiquitin molecules to substrate proteins, marking them for proteasomal degradation. UBE4B plays a role in elongating polyubiquitin chains, a process essential for efficient substrate recognition by the proteasome.
Research highlights its involvement in neurodegenerative diseases, such as Huntington’s disease, where impaired ubiquitination contributes to toxic protein aggregation. Additionally, UBE4B is implicated in cancer, acting as both an oncogene and tumor suppressor depending on context. Its interactions with p53 and EGFR pathways suggest roles in apoptosis and cell proliferation regulation.
UBE4B antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validated antibodies help explore UBE4B’s dual roles in disease mechanisms, therapeutic targeting, and its potential as a biomarker. Commercial antibodies are typically raised in rabbits or mice, targeting specific epitopes within UBE4B’s conserved regions. Proper validation, including knockout controls, ensures specificity, supporting studies on UPS dysregulation in pathological conditions.
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