首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Podocin酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NPHS2含量。NPHS2编码的蛋白podocin是肾小球裂孔隔膜的重要组成部分，对维持肾小球滤过屏障功能至关重要。研究机制方面，主要聚焦于其基因突变可导致足细胞功能障碍，引发先天性肾病综合征，为相关疾病的诊断和治疗提供了潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究肾小球损伤模型、足细胞功能调控实验或相关疾病分子标志物探索，为药物开发、病理机制分析及分子生物学研究提供定量检测支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：nephrosis 2 ELISA Kit; Nephrosis 2, idiopathic, steroid resistant (podocin) ELISA Kit; nephrosis 2, idiopathic, steroid resistant ELISA Kit; NPHS2 ELISA Kit; NPHS2 gene ELISA Kit; PDCN ELISA Kit; PODO_HUMAN ELISA Kit; Podocin ELISA Kit; SRN1 ELISA Kit
缩写：NPHS2
Uniprot No.：Q9NP85
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human podocin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 87 | |||||||||||
| Range % | 82-93 | ||||||||||||
| 1:2 | Average % | 93 | |||||||||||
| Range % | 84-99 | ||||||||||||
| 1:4 | Average % | 98 | |||||||||||
| Range % | 94-106 | ||||||||||||
| 1:8 | Average % | 93 | |||||||||||
| Range % | 86-98 | ||||||||||||
回收率：
| The recovery of human podocin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 91 | 84-96 | |||||||||||
| EDTA plasma (n=4) | 95 | 92-100 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected202.4932.5732.5332.418102.0762.0682.0721.95751.7031.7351.7191.6042.51.2711.2841.2781.1631.250.7890.8140.8020.6870.6250.4300.4650.4480.3330.3120.2610.2460.2540.13900.1160.1140.115 | |||||||||||||
货期：3-5 working days
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