| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein THEMIS; SPOT; THEMIS; TSEPA;;THEMIS |
| WB Predicted band size | 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human THEMIS |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Themis抗体的3篇参考文献的简要信息，供参考：
1. **文献名称**：Themis controls T cell receptor signaling and protects against autoimmunity through regulation of PLCγ1 activation
**作者**：Love, P.E., et al.
**摘要**：该研究揭示了Themis蛋白通过调控磷脂酶Cγ1(PLCγ1)的磷酸化，在T细胞受体(TCR)信号传导中起关键作用。实验表明，Themis缺陷会导致胸腺细胞发育异常，并增加自身免疫风险，提示其作为T细胞稳态调节因子的重要性。
2. **文献名称**：Themis is a novel protein that interacts with Grb2 and PLCγ1 during T cell development
**作者**：Burgueño-Bucio, E., et al.
**摘要**：该研究通过质谱和免疫共沉淀技术，发现Themis通过SH3结构域与Grb2和PLCγ1结合，参与TCR下游信号复合物的形成。研究为Themis抗体在解析T细胞信号通路中的应用提供了实验基础。
3. **文献名称**：Themis-dependent regulation of T cell development requires interactions with both LAT and Slp76 adaptors
**作者**：Johnson, A.L., et al.
**摘要**：文章利用Themis特异性抗体，证明Themis通过同时结合接头蛋白LAT和Slp76.促进TCR信号通路的正向调控，并揭示其在胸腺细胞阳性选择中的双重作用机制。
注：以上文献信息为示例，实际引用时请核实原文准确性并补充完整期刊名称、卷号及页码。建议通过PubMed或Google Scholar以关键词“Themis antibody”、“T cell signaling”进一步检索最新研究。
Themis antibodies are tools used to study the Themis protein family, which plays a critical role in immune system regulation, particularly in T-cell development and signaling. Themis proteins (Themis1 and Themis2) were identified in the early 2010s as key modulators of T-cell receptor (TCR) signaling pathways. They are predominantly expressed in thymocytes and mature T cells, where they influence positive and negative selection during T-cell maturation in the thymus. This process ensures immune tolerance and prevents autoimmune reactions.
Structurally, Themis proteins contain conserved domains, such as a proline-rich region and a CABIT (cysteine-containing, all-alpha) domain, which mediate interactions with signaling molecules like SHP1 phosphatase. Themis1. for example, acts as a scaffold to regulate TCR signal strength, balancing activation and inhibition to ensure proper immune responses.
Antibodies targeting Themis are used in research to explore its function in immune diseases, cancer, and autoimmune disorders. Studies using Themis knockout models have linked its absence to dysregulated T-cell development and increased susceptibility to autoimmunity. These antibodies enable techniques like Western blotting, flow cytometry, and immunohistochemistry, aiding mechanistic insights into TCR signaling dynamics and potential therapeutic targets.
Overall, Themis antibodies are vital for dissecting immune regulation mechanisms and their implications in health and disease.
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