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产品描述：人核仁磷酸蛋白(NPM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NPM1含量。NPM1是重要靶点。它在细胞生理过程中发挥关键作用，与核糖体合成、细胞周期调控等相关。研究发现其突变与急性髓系白血病发病紧密关联，相关机制研究多聚焦于其突变影响细胞内信号通路等，有望为白血病治疗提供新策略。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于白血病发病机制研究、实体瘤中NPM1异常表达的分子机制探索、药物干预效果评估等科研场景，例如通过血浆样本分析体外模型中的蛋白动态变化，或利用组织匀浆检测动物模型中NPM1的表达水平，亦可通过细胞裂解液评估基因编辑或药物处理后细胞内的蛋白表达差异。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B23 ELISA Kit; MGC104254 ELISA Kit; NO38 ELISA Kit; NPM ELISA Kit; NPM_HUMAN ELISA Kit; NPM1 ELISA Kit; Nucleolar phosphoprotein B23 ELISA Kit; Nucleolar protein NO38 ELISA Kit; Nucleophosmin (nucleolar phosphoprotein B23 numatrin) ELISA Kit; Nucleophosmin ELISA Kit; nucleophosmin nucleoplasmin family member 1 ELISA Kit; Nucleophosmin/nucleoplasmin family member 1 ELISA Kit; Numatrin ELISA Kit; OTTHUMP00000161024 ELISA Kit; OTTHUMP00000161025 ELISA Kit; OTTHUMP00000223397 ELISA Kit; OTTHUMP00000223398 ELISA Kit
缩写：NPM1
Uniprot No.：P06748
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NPM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human NPM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 97 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.3892.3802.3852.2397501.6041.5451.5751.4293751.1221.0681.0950.949187.50.7210.7330.7270.581940.4240.3900.4070.261470.3030.3010.3020.15623.50.2300.2370.2340.08800.1410.1500.146 | ||||||
货期：3-5 working days
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