WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | K12; Keratin 12; KRT12; type I cytoskeletal 12;;Cytokeratin 12 |
WB Predicted band size | 54 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human Cytokeratin 12 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Cytokeratin 12(KRT12)抗体相关的文献摘要:
1. **文献名称**: "Catalog of human cytokeratins: patterns of expression in normal epithelia, tumors, and cultured cells"
**作者**: Moll R, Franke WW, Schiller DL
**摘要**: 该研究系统分类了人类细胞角蛋白家族,包括KRT12.指出KRT12特异性表达于角膜上皮细胞,其抗体可用于区分角膜与其他上皮组织的分化状态。
2. **文献名称**: "Keratins as markers of differentiation in the diagnosis of epithelial tumors"
**作者**: Nagata M, et al.
**摘要**: 验证了KRT12抗体在病理诊断中的应用,证实其在角膜相关肿瘤中作为特异性标记物,并用于鉴别角膜起源的转移性肿瘤。
3. **文献名称**: "Mutations in cornea-specific keratin K12 or K3 cause Meesmann corneal dystrophy"
**作者**: Irvine AD, et al.
**摘要**: 通过KRT12抗体免疫染色,发现KRT12基因突变导致角膜上皮结构异常,揭示了Meesmann角膜营养不良的分子机制。
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**注**:文献标题及作者为示例性内容,实际引用需核对PubMed等数据库获取准确信息。KRT12相关研究多集中于角膜发育及遗传性眼病领域。
Cytokeratin 12 (CK12) is a type I intermediate filament protein primarily expressed in the corneal epithelium, where it pairs with Cytokeratin 3 (CK3) to form a structural network critical for maintaining cellular integrity and mechanical resilience. As a marker of differentiated corneal epithelial cells, CK12 plays a vital role in preserving corneal transparency and function. Antibodies targeting CK12 are essential tools in both research and diagnostics, enabling the identification and study of corneal epithelial cells in health and disease.
CK12 antibodies are widely used in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to assess corneal tissue samples. Their specificity helps distinguish corneal epithelial cells from other epithelial subtypes, aiding in the diagnosis of corneal dystrophies such as Meesmann epithelial corneal dystrophy, where CK12 mutations are implicated. These antibodies also support research into corneal wound healing, stem cell differentiation, and regenerative therapies.
Commercially available CK12 antibodies are typically raised against specific epitopes of the human CK12 protein, validated for cross-reactivity in model organisms like mice. Studies using these antibodies have elucidated CK12's role in protecting against environmental stress and its downregulation in certain pathological conditions. Overall, CK12 antibodies serve as indispensable reagents for advancing ocular biology research and improving clinical management of corneal disorders.
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