首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠诱导型一氧化氮合成酶(iNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOS2含量。NOS2即诱导型一氧化氮合酶，在炎症等病理状态下可被诱导表达。其背景在于在免疫防御等方面发挥重要作用。研究机制上，它能催化生成大量一氧化氮，参与调控炎症反应、细胞凋亡等过程，和多种疾病的发生发展关联紧密。试剂盒检测范围为0.78 IU/mL-50 IU/mL，为研究相关疾病模型的分子机制提供了可靠工具。检测体系覆盖0.78至50 IU/mL的线性范围，适用于评估实验动物模型中iNOS的动态变化，如炎症因子诱导的组织损伤研究、药物干预效果分析或免疫调节机制探索。通过检测iNOS的表达差异，可深入解析其在细胞信号通路中的作用，为心血管疾病、神经退行性病变等领域的机制研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nos2 ELISA Kit; Nitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
缩写：NOS2
Uniprot No.：Q06518
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 IU/mL-50 IU/mL
灵敏度：0.195 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of rat iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-95 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/ml.OD1OD2AverageCorrected502.2092.2672.2382.103251.5041.5341.5191.38412.50.9540.9610.9580.8236.250.5980.5750.5870.4523.120.3480.3690.3590.2241.560.2540.2670.2610.1260.780.2010.2110.2060.07100.1340.1360.135 | ||||||
货期：3-5 working days
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