| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA methyltransferase 1 associated protein 1; DNMAP1; DNMTAP1; EAF2; SWC4;;DMAP1 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DMAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DMAP1抗体的3篇参考文献示例(注：文献为虚拟示例，仅供参考格式)：
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1. **文献名称**: *"DMAP1 interacts with DNA methyltransferase 1 to regulate gene silencing and chromatin structure"*
**作者**: Rountree, M.R. et al.
**摘要**: 本研究揭示了DMAP1与DNMT1(DNA甲基转移酶1)的相互作用，利用DMAP1抗体通过免疫共沉淀(Co-IP)验证了两者的结合。实验表明DMAP1通过招募组蛋白去乙酰化酶(HDACs)参与异染色质形成和基因沉默，强调了其在表观遗传调控中的关键作用。
2. **文献名称**: *"DMAP1 modulates pluripotency in embryonic stem cells via histone acetylation dynamics"*
**作者**: Cui, Y. et al.
**摘要**: 通过免疫荧光和ChIP-seq技术结合DMAP1抗体，研究发现DMAP1通过调控组蛋白乙酰化水平影响胚胎干细胞的自我更新和分化。抗体特异性验证表明，DMAP1缺失导致多能性基因(如Oct4和Nanog)表达异常。
3. **文献名称**: *"DMAP1 as a potential biomarker in cancer: Antibody-based detection and functional analysis"*
**作者**: Saito, T. et al.
**摘要**: 该研究开发了一种高特异性DMAP1单克隆抗体，并用于Western blot和免疫组化分析。结果显示，DMAP1在多种癌症组织中高表达，且其表达水平与患者预后显著相关，提示其作为癌症治疗靶点的潜力。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“DMAP1 antibody”或“DMAP1 function”，并筛选涉及抗体应用的实验论文。
The DMAP1 (DNA methyltransferase 1-associated protein 1) antibody is a tool used to study the DMAP1 protein, a key regulatory factor involved in epigenetic modulation and chromatin remodeling. DMAP1 was initially identified as a binding partner of DNA methyltransferase 1 (DNMT1), an enzyme critical for maintaining DNA methylation patterns during replication. DMAP1 is implicated in diverse cellular processes, including transcriptional repression, cell cycle regulation, and DNA damage response. It functions as a component of the DNMT1 complex, facilitating the recruitment of DNMT1 to replication foci during S-phase to ensure proper maintenance of DNA methylation. Additionally, DMAP1 interacts with other chromatin-modifying complexes, such as the NuA3 histone acetyltransferase complex, suggesting roles in histone acetylation and chromatin structure modulation.
Antibodies targeting DMAP1 are widely used in molecular biology research to investigate its expression, localization, and interactions in various cell types. Applications include Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). These studies are critical for understanding DMAP1's involvement in epigenetic dysregulation linked to diseases like cancer, where aberrant DNA methylation and chromatin remodeling are common. DMAP1 antibodies are often validated for specificity using knockout cells or siRNA-mediated knockdown to confirm signal loss. Research utilizing these antibodies has expanded insights into how DMAP1 balances DNMT1 activity, impacts gene silencing, and contributes to cellular differentiation or oncogenesis, making it a focal point in epigenetics and cancer biology studies.
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