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产品描述：人内皮型一氧化氮合成酶(eNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NOS3含量。NOS3即内皮型一氧化氮合酶，主要在内皮细胞表达。其背景在于可催化L -精氨酸生成一氧化氮（NO）。研究机制方面，NO能舒张血管、抑制血小板聚集等。对NOS3的深入研究，有助于理解心血管生理病理过程，也为相关疾病治疗提供思路。试剂盒检测范围为1.1 IU/mL-70 IU/mL，在科学研究中，可应用于内皮细胞功能研究、心血管疾病模型评估、药物干预效果分析及体外实验中的分子机制探索，例如通过检测不同处理条件下细胞或动物模型中eNOS的动态变化，揭示其在氧化应激、炎症反应中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cNOS ELISA Kit; Constitutive NOS ELISA Kit; EC NOS ELISA Kit; EC-NOS ELISA Kit; ecNOS ELISA Kit; Endothelial nitric oxidase synthase ELISA Kit; Endothelial nitric oxide synthase ELISA Kit; Endothelial nitric oxide synthase 3 ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; Nitric oxide synthase 3 (endothelial cell) ELISA Kit; Nitric oxide synthase 3 ELISA Kit; Nitric oxide synthase 3 endothelial cell ELISA Kit; Nitric oxide synthase endothelial ELISA Kit; Nitric oxide synthase; endothelial ELISA Kit; NOS 3 ELISA Kit; NOS III ELISA Kit; NOS type III ELISA Kit; NOS3 ELISA Kit; NOS3_HUMAN ELISA Kit; NOSIII ELISA Kit
缩写：NOS3
Uniprot No.：P29474
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.1 IU/mL-70 IU/mL
灵敏度：0.27 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 81-102 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 84-115 | |||||
| 1:4 | Average % | 108 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 80-105 | |||||
回收率：
| The recovery of human eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 80-105 | ||||
| EDTA plasma (n=4) | 94 | 82-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected702.8352.6972.7662.612351.9301.9551.9431.78917.51.1461.1591.1530.9998.750.6730.7010.6870.5334.40.4570.4460.4520.2982.20.3210.3260.3240.1701.10.2430.2460.2450.09100.1560.1520.154 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human eNOS antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled eNOS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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