| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hSPT5; SPT5; SPT5H; supt5h; Tat CT1;;SPT5 |
| WB Predicted band size | Calculated MW: 121 kDa ; Observed MW: 155 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SPT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是几篇可能与SUPT5H抗体相关的参考文献示例(部分信息可能基于已有研究的概括，若需具体文献请通过学术数据库进一步检索)：
1. **文献名称**: "SUPT5H interacts with RNA polymerase II to coordinate transcriptional elongation and mRNA processing"
**作者**: Smith A, et al.
**摘要**: 该研究利用SUPT5H抗体进行染色质免疫沉淀(ChIP-seq)，揭示了SUPT5H蛋白与RNA聚合酶II的结合模式，证明其在转录延伸和RNA剪接偶联中的关键作用。
2. **文献名称**: "SUPT5H modulates HIV-1 transcription by recruiting p-TEFb complex"
**作者**: Lee J, et al.
**摘要**: 通过SUPT5H抗体的免疫共沉淀(Co-IP)实验，研究发现SUPT5H通过与p-TEFb复合物互作，调控HIV-1病毒的转录激活机制，为抗病毒治疗提供新靶点。
3. **文献名称**: "The role of SUPT5H in hepatocellular carcinoma progression"
**作者**: Zhang Y, et al.
**摘要**: 该研究使用SUPT5H抗体进行免疫组化(IHC)和Western blot分析，发现SUPT5H在肝癌组织中高表达，且其表达水平与患者预后不良相关，提示其作为潜在肿瘤标志物的可能性。
4. **文献名称**: "Genome-wide mapping of SUPT5H binding sites reveals its regulatory network"
**作者**: Chen L, et al.
**摘要**: 结合SUPT5H抗体的ChIP-seq数据与RNA测序，系统性鉴定了SUPT5H在全基因组范围内的结合位点，阐明其通过调控染色质可及性影响基因表达的分子机制。
**注意**：以上文献名称及内容为示例性概括，具体研究请以实际发表的论文为准。建议通过PubMed、Web of Science等平台以“SUPT5H antibody”或“SUPT5H function”为关键词检索最新文献。
The SUPT5H antibody targets the Suppressor of Ty 5 Homolog (SUPT5H) protein, also known as SPT5 or KOW, which is encoded by the SUPT5H gene in humans. This protein is a critical component of the SPT4/SPT5 complex, a transcription elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis. The SPT4/SPT5 heterodimer, part of the DRB sensitivity-inducing factor (DSIF), modulates transcriptional pausing, elongation efficiency, and termination, playing a dual role in promoting or inhibiting transcription depending on cellular context.
SUPT5H antibodies are widely used in molecular biology research to investigate transcriptional regulation mechanisms. They enable detection and quantification of SUPT5H expression via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These antibodies also facilitate studies on SUPT5H’s interaction with other transcriptional machinery components, such as P-TEFb or viral proteins like HIV-1 Tat, which hijack the SPT4/SPT5 complex for viral replication.
Research involving SUPT5H antibodies has shed light on its roles in diseases, including cancers (e.g., leukemia, solid tumors) and neurodevelopmental disorders linked to SUPT5H mutations. Additionally, studies explore its involvement in chromatin remodeling, DNA repair, and epigenetic regulation. Commercial SUPT5H antibodies are typically raised in rabbits or mice, validated for specificity across human and model organisms, and serve as essential tools for dissecting transcriptional dysregulation in health and disease.
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