首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经型一氧化氮合成酶(nNOS/NOS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的NOS1含量。NOS1即神经元型一氧化氮合酶，在神经系统中发挥重要作用。其背景在于它参与神经信号传导、调节血管张力等生理过程。研究机制上，主要聚焦于其催化产生一氧化氮的过程，以及该过程对神经功能、疾病发生发展的影响。试剂盒检测范围为31.25 mIU/mL-2000 mIU/mL，为神经科学、心血管疾病机制研究及药物开发等领域提供可靠的定量分析工具，特别适用于动物模型中nNOS表达水平与病理进程关联性研究、细胞模型信号通路分析等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nos1 ELISA Kit; BnosNitric oxide synthase ELISA Kit; brain ELISA Kit; EC 1.14.13.39 ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; NC-NOS ELISA Kit; NOS type I ELISA Kit; Neuronal NOS ELISA Kit; N-NOS ELISA Kit; nNOS ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit
缩写：NOS1
Uniprot No.：P29476
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 mIU/mL-2000 mIU/mL
灵敏度：7.81 mLU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat nNOS/NOS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of rat nNOS/NOS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-94 | ||||
| EDTA plasma (n=4) | 92 | 89-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mlU/mlOD1OD2AverageCorrected20002.1592.2562.2082.05510001.5871.6091.5981.4455001.2131.1561.1851.0322500.8440.8500.8470.6941250.4890.4650.4770.32462.50.2850.2770.2810.12831.250.2350.2530.2440.09100.1560.1500.153 | ||||||
货期：3-5 working days
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