首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人诱导型一氧化氮合成酶(iNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOS2含量。NOS2即诱导型一氧化氮合酶，可诱导生成大量一氧化氮。其背景在于参与炎症、免疫调节等生理病理过程。研究机制方面，主要聚焦其在疾病如炎症性疾病、肿瘤中的表达调控，以及它产生的一氧化氮对细胞功能、信号通路等的影响。试剂盒检测范围为0.9 IU/mL-60 IU/mL，适用于体外细胞实验、动物模型组织分析及生物标志物筛选等研究场景；为探索炎症机制、评估药物干预效果或疾病模型建立提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HEP-NOS ELISA Kit; Hepatocyte NOS ELISA Kit; HEPNOS ELISA Kit; inducible ELISA Kit; Inducible nitric oxide synthase ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; MAC NOS ELISA Kit; Macrophage NOS ELISA Kit; Nitric oxide synthase 2 inducible ELISA Kit; Nitric oxide synthase 2 inducible macrophage ELISA Kit; nitric oxide synthase 2A (inducible, hepatocytes) ELISA Kit; Nitric oxide synthase ELISA Kit; Nitric oxide synthase inducible ELISA Kit; nitric oxide synthase, macrophage ELISA Kit; NOS 2 ELISA Kit; NOS ELISA Kit; Nos II ELISA Kit; NOS type II ELISA Kit; nos2 ELISA Kit; NOS2_HUMAN ELISA Kit; NOS2A ELISA Kit; NOS2A, Inducible, Hepatocyte ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
缩写：NOS2
Uniprot No.：P35228
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 IU/mL-60 IU/mL
灵敏度：0.225 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-101 | |||||
回收率：
| The recovery of human iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected602.5192.4992.5092.378301.9341.8761.9051.774151.2771.2811.2791.1487.50.6950.6830.6890.5583.750.4420.4340.4380.3071.80.3170.3020.3100.1790.90.2180.2250.2220.09100.1330.1290.131 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human NOS2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NOS2 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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