首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经生长因子(NGF)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NGF含量。NGF即神经生长因子，是神经营养因子家族重要成员。它在神经元生长、发育、存活及功能维持中起关键作用。研究机制上主要包括与受体结合激活信号通路，调控基因表达等，其在神经系统疾病治疗等方面有重要研究价值。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于神经科学研究领域，包括大鼠脑损伤模型、神经炎症评估、疼痛机制探索及药物干预实验中NGF的动态监测，支持脑组织、坐骨神经等匀浆样本与体液样本的跨维度检测，为神经再生机制研究及化合物疗效评价提供可靠工具，满足基础科研中对低丰度蛋白的定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ngf ELISA Kit; Ngfb ELISA Kit; Beta-nerve growth factor ELISA Kit; Beta-NGF ELISA Kit
缩写：NGF
Uniprot No.：P25427
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of rat NGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected502.6352.5712.6032.477252.0522.1212.0871.96112.51.5631.5641.5641.4386.250.9170.9510.9340.8083.120.5020.4690.4860.3601.560.3340.3210.3280.2020.780.2410.2300.2360.11000.1280.1240.126 | ||||||
货期：3-5 working days
×
