首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Nerve growth factor,NGF ELISA kit

  • 中文名： 人神经生长因子(NGF)ELISA kit
货号: IPD-E04683h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人神经生长因子(NGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物.样本中的NGF含量。神经生长因子（NGF）是一种神经营养因子，对神经系统发育和功能至关重要。其研究机制涉及NGF与受体结合，激活信号通路，调节神经元生长、存活和功能。NGF在神经退行性疾病、疼痛管理等领域具有潜在治疗价值。试剂盒检测范围为6.86 pg/ml-5000 pg/ml.，灵敏度为6.86 pg/ml.。适用于神经生物学、疾病机制研究及药物开发等科研领域。通过本试剂盒可定量分析不同实验条件下（如疾病模型、药物处理或基因干预）样本中 NGF 的动态变化，为探究神经系统相关疾病的分子机制、筛选潜在治疗靶点或评估药物对神经保护作用提供可靠工具，适用于实验室常规分子生物学研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Beta nerve growth factor ELISA Kit; Beta NGF ELISA Kit; Beta-nerve growth factor ELISA Kit; Beta-NGF ELISA Kit; HSAN5 ELISA Kit; MGC161426 ELISA Kit; MGC161428 ELISA Kit; Nerve growth factor (beta polypeptide) ELISA Kit; Nerve growth factor ELISA Kit; Nerve growth factor beta ELISA Kit; Nerve growth factor beta polypeptide ELISA Kit; Nerve growth factor beta subunit ELISA Kit; NGF ELISA Kit; NGF_HUMAN ELISA Kit; NGFB ELISA Kit; NID67 ELISA Kit
  • 缩写：
    NGF
  • Uniprot No.：
    P01138
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
  • 检测范围：
    6.86 pg/ml-5000 pg/ml.
  • 灵敏度：
    6.86 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    90-100


    1:2

    Average %

    94


    Range %

    90-98


    1:4

    Average %

    92


    Range %

    87-100


    1:8

    Average %

    89


    Range %

    84-95


  • 回收率：

    The recovery of human NGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    85-97


    EDTA plasma (n=4)

    92

    88-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.813

    2.864

    2.839

    2.687


    1666.7

    2.346

    2.351

    2.349

    2.197


    555.6

    1.722

    1.752

    1.737

    1.585


    185.2

    1.133

    1.148

    1.141

    0.989


    61.7

    0.670

    0.683

    0.677

    0.525


    20.6

    0.483

    0.491

    0.487

    0.335


    6.86

    0.274

    0.283

    0.279

    0.127


    0

    0.150

    0.153

    0.152




  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×