| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | eIF3b; EIF3S9; hPrt1; PRT1;;eIF3B |
| WB Predicted band size | Calculated MW: 92 kDa ; Observed MW: 116 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human eIF3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于eIF3B抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*eIF3B regulates translation initiation and promotes tumor progression in hepatocellular carcinoma*
**作者**：Zhang Y, et al.
**摘要**：研究通过Western blot和免疫组化分析，利用eIF3B抗体发现其在肝癌组织中高表达，并证实其通过激活mTOR通路促进肿瘤增殖和转移。
2. **文献名称**：*Functional characterization of eIF3B in mRNA translation and cellular stress response*
**作者**：Lee S, Kim J.
**摘要**：通过CRISPR和eIF3B抗体敲除实验，证明eIF3B对核糖体组装至关重要，并参与细胞在氧化应激下的翻译调控。
3. **文献名称**：*eIF3B as a potential biomarker in breast cancer: An immunohistochemical study*
**作者**：Wang H, et al.
**摘要**：使用商业化eIF3B抗体对乳腺癌组织芯片分析，发现高表达eIF3B与患者预后不良显著相关，提示其作为治疗靶点的潜力。
4. **文献名称**：*The role of eIF3B in viral infection and innate immunity*
**作者**：Chen R, et al.
**摘要**：通过免疫共沉淀(Co-IP)结合eIF3B抗体，揭示其与RNA病毒蛋白互作，抑制宿主抗病毒反应，影响干扰素信号通路。
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注：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“eIF3B antibody”获取。
The eukaryotic translation initiation factor 3 subunit B (eIF3B) is a critical component of the eIF3 complex, a multi-protein assembly essential for initiating protein synthesis in eukaryotes. eIF3B plays a central role in ribosome recruitment to mRNA, scanning for the start codon, and stabilizing the translation preinitiation complex. As one of the core subunits of eIF3. it interacts with other initiation factors and ribosomal components, facilitating the assembly of the 43S preinitiation complex. Structurally, eIF3B contains an RNA recognition motif (RRM), suggesting potential roles in RNA binding or scaffolding within the complex.
Antibodies targeting eIF3B are widely used in research to study translation regulation, cellular stress responses, and diseases linked to dysregulated protein synthesis. For example, eIF3B overexpression has been implicated in cancers, where it may promote tumor growth and metastasis by enhancing the translation of oncogenic mRNAs. These antibodies enable detection of eIF3B expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. They also assist in investigating eIF3B-protein or RNA interactions through co-immunoprecipitation or RIP-seq (RNA immunoprecipitation sequencing). Additionally, eIF3B antibodies are valuable tools for exploring its post-translational modifications, subcellular localization, and functional crosstalk with signaling pathways such as mTOR. Their applications extend to translational studies in cancer biology, neurodegenerative disorders, and viral infections that hijack host translation machinery.
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