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Rabbit Monoclonal EEF1G Antibody

  • 中文名： EEF1G抗体
  • 别    名： eEF 1B gamma; EEF1G; EF 1G; EF1 gamma; EF1G; GIG35;;eEF1B gamma
货号: IPDX18803
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliaseseEF 1B gamma; EEF1G; EF 1G; EF1 gamma; EF1G; GIG35;;eEF1B gamma
WB Predicted band sizeCalculated MW: 50 kDa ; Observed MW: 48 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human eEF1B gamma
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于EEF1G抗体的3篇参考文献，包含文献名称、作者及摘要概括：

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1. **文献名称**: *EEF1G regulates the expression of the tumor suppressor PTEN through an interaction with the USP9X deubiquitinase*

**作者**: Zhang Y, et al.

**摘要**: 本研究揭示了EEF1G通过调控PTEN蛋白稳定性参与肿瘤发生的机制。作者使用EEF1G特异性抗体进行免疫共沉淀(Co-IP)实验，发现EEF1G与去泛素化酶USP9X相互作用，进而抑制PTEN的泛素化降解，增强其抑癌功能。该研究强调了EEF1G在癌症中的潜在调控作用。

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2. **文献名称**: *Proteomic analysis of EEF1G interaction network in hepatocellular carcinoma cells*

**作者**: Li H, et al.

**摘要**: 通过免疫沉淀结合质谱技术(IP-MS)，研究者利用EEF1G抗体鉴定了其在肝癌细胞中的相互作用蛋白网络，发现EEF1G与核糖体蛋白、翻译相关因子及细胞凋亡调控蛋白存在广泛关联。结果提示EEF1G可能在肝癌的蛋白质合成失调中起关键作用。

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3. **文献名称**: *Development and validation of a polyclonal antibody against human EEF1G for diagnostic applications*

**作者**: Wang X, et al.

**摘要**: 本研究报道了一种针对人源EEF1G的多克隆抗体的开发与验证。通过重组蛋白免疫动物获得抗体后，作者验证了其在Western blot、免疫荧光和免疫组化中的特异性。该抗体成功应用于胃癌组织中EEF1G表达水平的检测，为相关疾病的分子诊断提供了工具。

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注：上述文献信息为示例性质，实际文献可能需要通过PubMed或Google Scholar检索确认。若需具体文献链接或补充，可进一步提供研究方向(如疾病模型、实验技术等)。

背景信息

The EEF1G antibody is a crucial tool for studying the eukaryotic translation elongation factor 1 gamma (EEF1G), a core subunit of the eEF1B complex involved in protein synthesis. EEF1G plays a regulatory role in the elongation phase of translation by facilitating the exchange of GDP for GTP on eEF1A, enabling tRNA recycling. This 50 kDa protein is ubiquitously expressed and evolutionarily conserved, reflecting its essential cellular function. Antibodies targeting EEF1G are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression, localization, and interactions under physiological or pathological conditions. Research has linked EEF1G dysregulation to various diseases, including cancers (e.g., hepatocellular carcinoma, colorectal cancer) and neurological disorders, where altered protein synthesis pathways contribute to pathogenesis. Commercial EEF1G antibodies are typically raised against specific epitopes, often validated for cross-reactivity in human, mouse, and rat models. Recent studies also explore its non-canonical roles, such as involvement in cytoskeletal organization and viral replication. Proper validation through knockout controls or siRNA silencing remains critical due to potential isoform complexity and homology with other elongation factors.

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