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产品描述：人核因子红细胞2相关因子2(NFE2L2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆、细胞裂解物样本中的NFE2L2含量。NFE2L2是重要靶点。它是细胞氧化应激反应的关键转录因子，维持体内氧化还原平衡。研究发现其异常激活或抑制与多种疾病如癌症、神经退行性疾病等密切相关，机制涉及多条信号通路，有望为相关疾病治疗提供新方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒通过高特异性配对抗体实现靶标捕获与信号放大，适用于基础科研领域中的氧化应激相关机制研究、药物开发中的药效评估以及环境毒理学中的生物标志物分析等场景，能够为体外模型、动物实验或人体样本中的NFE2L2动态变化提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：erythroid derived 2 ELISA Kit; HEBP1 ELISA Kit; like 2 ELISA Kit; NF E2 related factor 2 ELISA Kit; NF-E2-related factor 2 ELISA Kit; NF2L2_HUMAN ELISA Kit; NFE2 related factor 2 ELISA Kit; NFE2-related factor 2 ELISA Kit; Nfe2l2 ELISA Kit; Nrf 2 ELISA Kit; NRF2 ELISA Kit; Nuclear factor (erythroid derived 2) like 2 ELISA Kit; Nuclear factor ELISA Kit; nuclear factor erythroid 2 like 2 ELISA Kit; Nuclear factor erythroid 2 related factor 2 ELISA Kit; Nuclear factor erythroid 2-related factor 2 ELISA Kit; Nuclear factor erythroid derived 2 like 2 ELISA Kit
缩写：NFE2L2
Uniprot No.：Q16236
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NFE2L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human NFE2L2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-104 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3362.3522.3442.18710001.7871.7761.7821.6255001.2801.2541.2671.1102500.8450.8210.8330.6761250.5710.5840.5780.42162.50.3640.3590.3620.20531.250.2410.2540.2480.09100.1560.1580.157 | ||||||
货期：3-5 working days
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