首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人唾液酸酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、脑脊液（CSF）样本中的NEU1含量。NEU1是一种神经氨酸酶，主要在溶酶体中发挥功能，负责从糖蛋白和糖脂中去除硅酸残基。其失调与多种疾病相关，包括溶酶体储存疾病、自身免疫疾病和癌症。NEU1在细胞信号传导、免疫反应和病原体识别中起重要作用，其研究有助于揭示疾病机制和开发新治疗策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可应用于基础研究领域，如探究唾液酸酶在感染性疾病、神经系统疾病中的生物学功能，或评估其在体液中的动态变化规律，为疾病机制研究及生物标志物筛选提供可靠工具。试剂盒兼容血清、尿液及CSF等复杂体液样本，具备良好的稳定性和重复性，适用于实验室级科研分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acetylneuraminyl hydrolase ELISA Kit; exo-alpha-sialidase ELISA Kit; G9 sialidase ELISA Kit; Lysosomal sialidase ELISA Kit; N acetyl alpha neuraminidase 1 ELISA Kit; N-acetyl-alpha-neuraminidase 1 ELISA Kit; NANH ELISA Kit; NEU ELISA Kit; NEU1 ELISA Kit; NEUR1_HUMAN ELISA Kit; Neuraminidase 1 ELISA Kit; Neuraminidase ELISA Kit; SIAL1 ELISA Kit; sialidase 1 (lysosomal sialidase) ELISA Kit; Sialidase 1 ELISA Kit; Sialidase; lysosomal ELISA Kit; Sialidase-1 ELISA Kit
缩写：NEU1
Uniprot No.：Q99519
种属：Homo sapiens (Human)
样本类型：serum, urine, cerebrospinal fluid (CSF)
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sialidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human sialidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6212.5222.5722.460102.2122.1122.1622.05051.7011.6541.6781.5662.51.2571.1971.2271.1151.250.7630.7410.7520.6400.6250.4610.4340.4480.3360.3120.2870.2730.2800.16800.1150.1090.112 | ||||||
货期：3-5 working days
×
