首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人中性内肽酶/脑啡肽酶(NEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NEP含量。NEP即中性内肽酶，是一种重要的膜结合金属蛋白酶。其背景在于它在体内参与多种生物活性肽的降解。研究机制方面，它能通过特异性切割底物的肽键发挥作用，在心血管、肾脏等疾病病理生理过程及药物研发中是关键靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外定量分析NEP在基础科研中的表达特征，例如神经系统疾病模型研究、心血管代谢调控机制探索、肿瘤微环境相关肽类信号通路分析等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Atriopeptidase ELISA Kit; CALLA ELISA Kit; CD10 ELISA Kit; CD10 antigen ELISA Kit; Common acute lymphocytic leukemia antigen ELISA Kit; DKFZp686O16152 ELISA Kit; EC 3.4.24.11 ELISA Kit; Enkephalinase ELISA Kit; EPN ELISA Kit; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) ELISA Kit; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) ELISA Kit; Membrane metallo endopeptidase ELISA Kit; Membrane metallo endopeptidase variant 1 ELISA Kit; Membrane metallo endopeptidase variant 2 ELISA Kit; Membrane metalloendopeptidase ELISA Kit; Membrane metalloendopeptidase neutral endopeptidase enkephalinase ELISA Kit; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 ELISA Kit; Membrane metalloendopeptidase variant 1 ELISA Kit; Membrane metalloendopeptidase variant 2 ELISA Kit; MGC126681 ELISA Kit; MGC126707 ELISA Kit; MME ELISA Kit; NEP ELISA Kit; NEP_HUMAN ELISA Kit; Neprilysin ELISA Kit; neprilysin-390 ELISA Kit; neprilysin-411 ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; Neutral endopeptidase ELISA Kit; Neutral endopeptidase, membrane-associated ELISA Kit; SFE ELISA Kit; Skin fibroblast elastase ELISA Kit
缩写：NEP
Uniprot No.：P08473
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-109 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-93 | |||||
回收率：
| The recovery of human NEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.3882.5802.4842.340 25001.8361.9471.8921.748 12501.4711.4251.4481.304 6250.9251.0050.9650.821 312.50.6050.6540.6300.486 1560.3880.3890.3890.245 780.2490.2440.2470.103 00.1380.1490.144 | ||||||
货期：3-5 working days
×
