首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人再生蛋白(NEO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NEO1含量。NEO1 即神经纤毛蛋白 - 1，它属于细胞表面受体，在胚胎发育、血管生成等生理过程中发挥重要作用。其研究机制主要聚焦在与配体相互作用，调节细胞信号通路，进而影响细胞的增殖、迁移和存活，目前在肿瘤等疾病的治疗研究中受关注。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品适用于体外研究NEO1在细胞迁移、组织修复模型中的调控机制，或用于评估药物干预、基因编辑等实验条件下目标蛋白的表达特征，为再生医学基础研究、疾病模型构建及生物标志物探索提供精准的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Immunoglobulin superfamily DCC subclass member 2 ELISA Kit; NEO1 ELISA Kit; NEO1_HUMAN ELISA Kit; Neogenin ELISA Kit; Ngn (gene name) ELISA Kit
缩写：NEO1
Uniprot No.：Q92859
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NEO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 99 | |||||
| Range % | 94-104 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 83-97 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-97 | ||||||
| 1:8 | Average % | 111 | |||||
| Range % | 106-114 | ||||||
回收率：
| The recovery of human NEO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-99 | |||||
| EDTA plasma (n=4) | 105 | 103-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| vpg/mlOD1OD2AverageCorrected50002.0842.0202.0521.93325001.4031.4541.4291.31012501.0511.0981.0750.9566250.7120.7210.7170.5983120.3580.3650.3620.2431560.2450.2540.2500.131780.1770.1780.1780.05900.1150.1220.119 | |||||||
货期：3-5 working days
×
