| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASK; CHIF; DBF4; DBF4 zinc finger A; DBF4A; ZDBF1;;DBF4 |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human DBF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DBF4抗体的参考文献示例(内容为虚构，仅供格式参考，建议通过学术数据库查询真实文献)：
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1. **"DBF4-CDC7 kinase complex in DNA replication initiation"**
*作者：Kumagai A., Dunphy W.G.*
摘要：研究DBF4与CDC7激酶复合体在DNA复制起始中的作用，利用特异性抗体证明其在细胞周期S期的调控机制。
2. **"Targeting DBF4 as a therapeutic strategy in cancer"**
*作者：Montagnoli A., et al.*
摘要：通过DBF4抗体检测肿瘤细胞中DBF4过表达现象，提出抑制DBF4-CDC7复合物可阻断癌细胞增殖。
3. **"Development of a monoclonal antibody for human DBF4 protein"**
*作者：Rodriguez-Acebes S., et al.*
摘要：报道一种高特异性抗人DBF4单克隆抗体的开发，验证其在免疫印迹和免疫荧光中的应用效果。
4. **"DBF4 expression correlates with replication stress in colorectal cancer"**
*作者：Matson J.P., Cortez D.*
摘要：使用DBF4抗体分析结直肠癌样本，发现DBF4表达水平与DNA复制应激及化疗敏感性相关。
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建议通过 **PubMed** 或 **Google Scholar** 搜索关键词“DBF4 antibody”“CDC7-DBF4 complex”获取真实文献。
The DBF4 antibody is a research tool designed to detect and study the DBF4 protein, a key regulatory component in eukaryotic DNA replication. DBF4 (Dbf4-dependent kinase subunit) serves as the regulatory partner of the CDC7 kinase, forming the CDC7-DBF4 complex (also known as the DBF4-dependent kinase, DDK). This complex is essential for initiating DNA replication by phosphorylating subunits of the MCM (minichromosome maintenance) helicase, enabling the unwinding of DNA at replication origins during the G1/S phase transition of the cell cycle. DBF4 expression is tightly cell cycle-regulated, peaking during S phase and declining thereafter.
Antibodies targeting DBF4 are widely used in molecular and cell biology to investigate replication initiation mechanisms, cell cycle progression, and checkpoint responses. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze DBF4 protein levels, localization, and interactions. Dysregulation of DBF4 has been implicated in genomic instability and cancer, making these antibodies valuable in oncology research. For example, studies use DBF4 antibodies to explore overexpression in tumors or its role in replication stress responses. Commercial DBF4 antibodies are typically raised against conserved epitopes in human or model organisms (e.g., yeast, mice), with validation in specific experimental systems. Researchers must verify cross-reactivity and optimize protocols for their models.
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