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产品描述：人蛋白激酶C结合蛋白NELL1(NELL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NELL1含量。NELL1蛋白是一种新型骨诱导生长因子，与人颅缝早闭症相关，能有效促进骨生长。其研究机制主要涉及促进干细胞分化、诱导骨形成、抑制脂肪分化和炎症反应，并与骨形态发生蛋白2等协同作用，提高骨再生安全性和有效性。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，其多样本兼容性支持科研人员探索NELL1在骨骼生物学、干细胞分化调控、创伤修复机制等领域的表达特征，尤其适用于骨代谢疾病模型、组织工程研究及药物干预实验中的蛋白水平监测，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ45906 ELISA Kit; IDH3GL ELISA Kit; NEL like 1 (chicken) ELISA Kit; NEL like protein 1 ELISA Kit; Nel related protein 1 ELISA Kit; NEL-like 1 (chicken) ELISA Kit; NEL-like protein 1 ELISA Kit; Nel-related protein 1 ELISA Kit; NELL 1 protein short isoform ELISA Kit; Nell1 ELISA Kit; NELL1_HUMAN ELISA Kit; Neural epidermal growth factor like 1 ELISA Kit; NRP1 ELISA Kit; Protein kinase C binding protein NELL1 ELISA Kit; Protein kinase C-binding protein NELL1 ELISA Kit
缩写：NELL1
Uniprot No.：Q92832
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NELL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:10 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human NELL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.9072.9872.9472.768 10002.2602.2082.2342.055 5001.4281.4351.4321.253 2500.8410.8650.8530.674 1250.5380.5680.5530.374 62.50.3980.3770.3880.209 31.250.2980.2710.2850.106 00.1830.1750.179 | ||||||
货期：3-5 working days
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