首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人核仁蛋白(NCL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NCL含量。核仁素（NCL）是一种多功能蛋白质，广泛存在于真核细胞中。它参与了核糖体合成、染色质稳定性维持等重要细胞过程。在疾病研究中，NCL与肿瘤发生发展密切相关，可能通过调控基因表达、促进肿瘤细胞增殖和转移等机制发挥作用，是潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于肿瘤分子机制研究、药物靶点筛选、细胞增殖与凋亡相关实验等科研场景，特别适合体外培养细胞模型或动物实验样本中NCL的功能探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C23 ELISA Kit; FLJ45706 ELISA Kit; MS1116 ELISA Kit; NCL ELISA Kit; Nucl ELISA Kit; NUCL_HUMAN ELISA Kit; Nucleolin ELISA Kit; Protein C23 ELISA Kit
缩写：NCL
Uniprot No.：P19338
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NCL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human NCL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6752.6562.6662.54210002.3122.4561.9941.8705001.7091.6831.4401.3162501.0470.9980.9220.7981250.6170.5890.4960.37262.50.4290.4030.4150.29131.250.2380.2210.2300.10600.1290.1190.124 | ||||||
货期：3-5 working days
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