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产品描述：人神经母细胞瘤致瘤性抑制因子1(NBL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NBL1含量。试剂盒检测范围为34.4 pg/mL-2200 pg/mL，该产品适用于体外培养细胞模型、动物组织样本及体液标本的NBL1蛋白表达量检测，可支持肿瘤生物学、神经科学领域的基础研究，以及药物干预实验中生物标志物的动态监测，为探索NBL1在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D1S1733E ELISA Kit; DAN ELISA Kit; DAN domain family member 1 ELISA Kit; DAND 1 ELISA Kit; DAND1 ELISA Kit; Differential screening selected gene aberrant in neuroblastoma ELISA Kit; MGC8972 ELISA Kit; NB ELISA Kit; NBL 1 ELISA Kit; NBL1 ELISA Kit; NBL1_HUMAN ELISA Kit; Neuroblastoma candidate region suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1 ELISA Kit; NO 3 ELISA Kit; NO3 ELISA Kit; Protein N03 ELISA Kit; Zinc finger protein DAN ELISA Kit
缩写：NBL1
Uniprot No.：P41271
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：34.4 pg/mL-2200 pg/mL
灵敏度：8.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NBL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 84-96 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 97-107 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 83-94 | ||||||
回收率：
| The recovery of human NBL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 94-105 | |||||
| EDTA plasma (n=4) | 106 | 101-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected22001.7551.8211.7881.66511001.2851.2891.2871.1645500.8480.8450.8470.7242750.5470.5330.5400.417137.50.3530.3650.3590.23668.750.2980.3210.3100.18734.40.2250.2140.2200.09700.1250.1210.123 | |||||||
货期：3-5 working days
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