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产品描述：人天冬氨酸蛋白酶A(NAPSA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NAPSA含量。人天冬氨酸蛋白酶A（NAPSA）是一种在细胞凋亡和肿瘤发生中发挥关键作用的蛋白酶。其研究机制涉及其在细胞内的相互作用、催化活性以及与底物的特异性结合。NAPSA通过切割特定肽键来调控细胞进程，其抑制剂作为潜在治疗剂备受关注。试剂盒检测范围为4.7 ng/mL-300 ng/mL，科研人员可利用定量分析体液或组织样本中的NAPSA浓度变化，应用于肺发育生物学研究、肿瘤微环境分析、疾病机制探索等领域，为研究该蛋白在细胞分化、组织修复及病理状态下的调控作用提供实验工具。试剂盒操作流程标准化，配套实验方案详实，适用于生命科学基础研究与生物标志物探索等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Asp 4 ELISA Kit; ASP4 ELISA Kit; Aspartyl protease 4 ELISA Kit; KAP ELISA Kit; Kdap ELISA Kit; Kidney derived aspartic protease like protein ELISA Kit; NAP1 ELISA Kit; NAPA ELISA Kit; Napsa ELISA Kit; NAPSA_HUMAN ELISA Kit; Napsin 1 ELISA Kit; napsin A aspartic peptidase ELISA Kit; Napsin A precursor ELISA Kit; Napsin-1 ELISA Kit; Napsin-A ELISA Kit; Pronapsin A ELISA Kit; SNAPA ELISA Kit; TA01/TA02 ELISA Kit
缩写：NAPSA
Uniprot No.：O96009
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 ng/mL-300 ng/mL
灵敏度：1.18 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NAPSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:10 | Average % | 101 | |||||
| Range % | 95-104 | ||||||
| 1:20 | Average % | 97 | |||||
| Range % | 89-101 | ||||||
| 1:40 | Average % | 106 | |||||
| Range % | 98-110 | ||||||
回收率：
| The recovery of human NAPSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 95-104 | |||||
| EDTA plasma (n=4) | 101 | 94-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected3002.4352.5272.4812.2891502.0422.1042.0731.881751.4551.4091.4321.24037.50.8430.8230.8330.64118.750.5520.5380.5450.3539.40.3810.3760.3790.1874.70.3010.3110.3060.11400.1900.1940.192 | |||||||
货期：3-5 working days
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