| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hTIM; TIM; TIM1; Timeless; timeless circadian clock 1; TIMELESS1;;Timeless |
| WB Predicted band size | Calculated MW: 139 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Timeless |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Timeless抗体的3篇代表性文献摘要概括(部分为模拟示例，实际文献需通过数据库验证)：
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1. **文献名称**: "Timeless interacts with the circadian clock and regulates DNA replication in mammalian cells"
**作者**: Lee, C., & Belmont, A. S.
**摘要**: 本研究首次报道了哺乳动物Timeless蛋白在生物钟调控与DNA复制中的双重功能。作者通过制备特异性Timeless抗体，证实其与CRY蛋白的相互作用，并利用ChIP实验揭示Timeless在复制起点上的周期性富集，为理解昼夜节律与基因组稳定性关联提供机制依据。
2. **文献名称**: "A human homolog of the Drosophila Timeless gene involved in CHK1-mediated S phase checkpoint"
**作者**: Unsal-Kacmaz, K., & Vindigni, A.
**摘要**: 文章鉴定人类Timeless(hTIM)作为DNA损伤应答的关键因子。研究团队开发了针对hTIM的单克隆抗体，通过免疫沉淀和蛋白互作分析，证明hTIM与CHK1激酶协同调控S期检查点，提示其作为抗癌靶点的潜力。
3. **文献名称**: "Antibody-based profiling of circadian clock proteins in mammalian tissues"
**作者**: Koike, N., & Rosbash, M.
**摘要**: 该研究系统评估了多种生物钟相关抗体(包括Timeless)的特异性与适用性。通过Western blot和免疫组化分析，揭示Timeless蛋白在小鼠肝脏中的振荡表达模式，为昼夜节律研究提供可靠检测工具。
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**注**：以上文献为领域内典型研究方向模拟，实际引用请通过PubMed/Google Scholar检索关键词“Timeless antibody”、“hTIM function”等，优先选择近5年高被引论文或权威综述。部分经典研究可参考：
- Unsal-Kacmaz et al. *Mol Cell Biol* (2005) 对Timeless抗体的早期应用
- Yoshizawa-Sugata et al. *J Biol Chem* (2007) 解析Timeless抗体在复制应激中的检测方法
**Background of Timeless Antibody**
The Timeless protein, a conserved component of the DNA replication machinery, plays a critical role in maintaining genome stability by facilitating DNA replication and repair. It interacts with partners like TIPIN and Claspin to form the replication fork protection complex, which stabilizes stalled replication forks during replication stress. Dysregulation of Timeless is linked to genomic instability, a hallmark of cancer and other diseases.
Timeless antibodies are essential tools for studying its function, localization, and expression in cellular contexts. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate Timeless' role in cell cycle regulation, replication fork dynamics, and the DNA damage response. Elevated Timeless levels have been observed in various cancers, correlating with poor prognosis, suggesting its potential as a therapeutic target or biomarker.
Studies leveraging Timeless antibodies have also explored its interplay with checkpoint kinases (e.g., ATR, Chk1) and its involvement in circadian rhythm regulation, hinting at broader physiological impacts. Ongoing research aims to clarify its mechanisms in tumorigenesis and exploit Timeless inhibition for cancer therapy.
In summary, Timeless antibodies are pivotal for unraveling the protein's contributions to genome integrity and disease, offering insights into targeted treatment strategies.
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