| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Desmoplakin I; Desmoplakin II; DPI; DPII; DSP; KPPS2; PPKS2;;Desmoplakin |
| WB Predicted band size | Calculated MW: 332 kDa ; Observed MW: 270 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human Desmoplakin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于Desmoplakin抗体的3篇示例文献(仅供参考，实际文献需通过学术数据库查询)：
---
1. **文献名称**：*"Desmoplakin antibodies in paraneoplastic pemphigus"*
**作者**：Joly, P. et al.
**摘要**：研究报道副肿瘤性天疱疮患者血清中存在的抗Desmoplakin抗体，通过免疫沉淀和免疫印迹技术证实其与表皮桥粒结构破坏相关，提示其在皮肤损伤中的作用机制。
2. **文献名称**：*"Structural analysis of desmoplakin domains using cryo-electron microscopy"*
**作者**：Al-Jassar, C. et al.
**摘要**：利用冷冻电镜解析Desmoplakin蛋白的N端结构域，阐明其与中间丝蛋白相互作用的分子机制，为抗体在桥粒组装中的功能提供结构基础。
3. **文献名称**：*"Desmoplakin mutations and arrhythmogenic cardiomyopathy"*
**作者**：Norgett, E.E. et al.
**摘要**：发现Desmoplakin基因突变与致心律失常性右室心肌病(ARVC)相关，免疫组化显示心肌细胞间连接异常，提示抗体检测可用于疾病诊断。
---
**注**：以上为示例，实际文献需通过PubMed、Google Scholar等平台以关键词“Desmoplakin antibody”或“Desmoplakin autoantibody”检索，并筛选应用或机制相关研究。
**Background of Desmoplakin Antibodies**
Desmoplakin (DSP) is a critical component of desmosomes, specialized intercellular junctions that maintain structural integrity and mechanical stability in tissues subjected to stress, such as the skin, heart, and epithelial layers. As a member of the plakin protein family, desmoplakin links intermediate filaments (e.g., keratins, desmin) to desmosomal cadherins, ensuring cell-cell adhesion and tissue cohesion.
Antibodies targeting desmoplakin are widely used in research and diagnostics to study desmosome assembly, function, and related pathologies. In autoimmune disorders like paraneoplastic pemphigus or pemphigus vulgaris, anti-desmoplakin autoantibodies may contribute to tissue blistering by disrupting desmosomal adhesion. Additionally, mutations in the *DSP* gene are linked to hereditary conditions such as arrhythmogenic cardiomyopathy (AC) and skin disorders like Carvajal syndrome. Desmoplakin antibodies help identify abnormal protein expression or localization in these diseases, aiding in molecular diagnosis and mechanistic studies.
In cancer research, altered desmoplakin expression correlates with tumor progression and metastasis, as disrupted cell adhesion often promotes invasive behavior. These antibodies are also essential tools in immunohistochemistry, Western blotting, and immunofluorescence to visualize desmosomal structures in tissue samples. Overall, desmoplakin antibodies play a pivotal role in elucidating cellular adhesion mechanisms and their implications in genetic, autoimmune, and neoplastic diseases.
×
