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产品描述：人N-myc原癌蛋白(MYCN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYCN含量。MYCN 是一个重要的靶点。它属于 MYC 基因家族，与神经母细胞瘤等肿瘤发生发展密切相关。其编码的蛋白是转录因子，可调控细胞增殖、分化和凋亡等。当前针对 MYCN 的研究机制主要聚焦于如何抑制其活性以阻断肿瘤生长。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域探究肿瘤发生机制、评估抗癌药物对MYCN表达的影响，或通过体外模型研究基因调控网络，为肿瘤生物学研究提供可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bHLHe37 ELISA Kit; Class E basic helix-loop-helix protein 37 ELISA Kit; MODED ELISA Kit; mycn ELISA Kit; MYCN_HUMAN ELISA Kit; N myc ELISA Kit; N myc proto oncogene protein ELISA Kit; N-myc proto-oncogene protein ELISA Kit; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene ELISA Kit; Neuroblastoma MYC oncogene ELISA Kit; NMYC ELISA Kit; NMYC proto oncogene protein ELISA Kit; ODED ELISA Kit; Oncogene NMYC ELISA Kit; pp65/67 ELISA Kit; V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog ELISA Kit; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived ELISA Kit; v myc myelocytomatosis viral related oncogene neuroblastoma derived ELISA Kit
缩写：MYCN
Uniprot No.：P04198
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MYCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 99-108 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MYCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-101 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4142.3282.3712.2135001.3971.4921.4451.2872500.8970.8570.8770.7191250.5770.5680.5730.41562.50.3750.3640.3700.21231.20.2810.2960.2890.13115.60.2280.2150.2220.06400.1590.1570.158 | ||||||
货期：3-5 working days
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