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产品描述：人基质重塑关联蛋白8(MXRA8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MXRA8含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，支持科研人员开展疾病模型（如纤维化疾病、病毒感染或肿瘤微环境等）中基质重塑相关机制研究；可用于评估不同处理条件下组织样本中MXRA8的动态变化；适用于基础医学、分子生物学及病理学领域的科学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Limitrin ELISA Kit; Matrix remodelling associated 8 ELISA Kit; Matrix-remodeling-associated protein 8 ELISA Kit; Mxra8 ELISA Kit; MXRA8_HUMAN ELISA Kit
缩写：MXRA8
Uniprot No.：Q9BRK3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MXRA8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 81-92 | ||||||
| 1:2 | Average % | 109 | |||||
| Range % | 101-113 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 83-95 | ||||||
回收率：
| The recovery of human MXRA8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 82-94 | |||||
| EDTA plasma (n=4) | 103 | 94-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.3062.2172.2622.15051.5431.5231.5331.4212.50.9521.0010.9770.8651.250.6190.6000.6100.4980.6250.3630.3430.3530.2410.3120.2590.2520.2560.1440.1560.1740.1810.1780.06600.1100.1140.112 | |||||||
货期：3-5 working days
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