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产品描述:人c-myc癌基因产物(c-myc)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYC含量。MYC是重要靶点。它是原癌基因,在细胞增殖、分化及凋亡中起关键作用。异常激活与多种癌症发生发展密切相关。研究聚焦其调控机制,探索抑制途径,旨在开发针对MYC的抗癌疗法,为癌症治疗带来新可能。试剂盒检测范围为0.312 ng/mL-20 ng/mL,该产品适用于肿瘤生物学研究、药物作用机制探索、细胞周期调控分析等科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:AU016757 ELISA Kit; Avian myelocytomatosis viral oncogene homolog ELISA Kit; bHLHe39 ELISA Kit; c Myc ELISA Kit; Cellular myelocytomatosis oncogene ELISA Kit; Class E basic helix-loop-helix protein 39 ELISA Kit; MGC105490 ELISA Kit; MRTL ELISA Kit; Myc ELISA Kit; Myc protein ELISA Kit; Myc proto oncogene protein ELISA Kit; Myc proto-oncogene protein ELISA Kit; myc-related translation/localization regulatory factor ELISA Kit; MYC_HUMAN ELISA Kit; Myc2 ELISA Kit; myca ELISA Kit; MYCC ELISA Kit; Myelocytomatosis oncogene a ELISA Kit; Myelocytomatosis oncogene ELISA Kit; Niard ELISA Kit; Nird ELISA Kit; oncogene c-Myc ELISA Kit; Oncogene Myc ELISA Kit; OTTHUMP00000158589 ELISA Kit; OTTHUMP00000227763 ELISA Kit; Proto-oncogene c-Myc ELISA Kit; Protooncogene homologous to myelocytomatosis virus ELISA Kit; RNCMYC ELISA Kit; Transcription factor p64 ELISA Kit; Transcriptional regulator Myc-A ELISA Kit; V-Myc avian myelocytomatosis viral oncogene homolog ELISA Kit; v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit; zc-myc ELISA Kit
缩写:MYC
Uniprot No.:P01106
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:0.312 ng/mL-20 ng/mL
灵敏度:0.078 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human c-myc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 87-99 | |||||
1:2 | Average % | 95 | ||||
Range % | 91-101 | |||||
1:4 | Average % | 92 | ||||
Range % | 88-96 | |||||
1:8 | Average % | 90 | ||||
Range % | 86-97 |
回收率:
The recovery of human c-myc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 85-96 | ||||
EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected202.4042.4532.4292.341101.5871.5751.5811.49351.0841.0661.0750.9872.50.6580.6990.6790.5911.250.4560.4870.4720.3840.6250.2510.2450.2480.1600.3120.1610.1690.1650.07700.0870.0890.088 |
货期:3-5 working days
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