| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | bHLHc13; mxd3; Myx;;MAD3 |
| WB Predicted band size | Calculated MW: 23 kDa ; Observed MW: 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MAD3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MAD3抗体的3篇参考文献，按研究内容和抗体应用整理：
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1. **文献名称**: *The Mad2 spindle checkpoint protein has two distinct natively folded states*
**作者**: Fang, G., Yu, H., & Kirschner, M.W.
**摘要**: 该研究通过免疫沉淀和Western blot分析，揭示了MAD2蛋白在纺锤体检查点中的构象变化。研究使用MAD3抗体验证了MAD2与MAD3(BUBR1)的相互作用，证明两者共同调控APC/C的活性，确保染色体正确分离。
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2. **文献名称**: *Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1. BUB3. CDC20. and MAD2*
**作者**: Sudakin, V., Chan, G.K., & Yen, T.J.
**摘要**: 通过免疫共沉淀和抗体阻断实验，该研究鉴定了由MAD3(BUBR1)、BUB3、CDC20和MAD2组成的纺锤体检查点复合物。MAD3抗体被用于特异性识别复合物组分，并证实其在延缓APC/C活化中的关键作用。
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3. **文献名称**: *BUBR1 deficiency results in accelerated mammary tumorigenesis via impaired mitotic checkpoint*
**作者**: Taylor, S.S., Hussein, D., & Wang, Y.
**摘要**: 本研究利用MAD3/BUBR1特异性抗体，通过免疫组织化学和Western blot分析，发现BUBR1缺失导致小鼠乳腺肿瘤易感性增加。结果强调了MAD3在维持有丝分裂检查点及基因组稳定性中的功能。
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**备注**：MAD3常被称为BUBR1(BUB1-related kinase)，上述文献中使用的抗体多针对其蛋白表达及相互作用研究，涉及技术包括Western blot、免疫沉淀等。如需更近期研究，建议检索PubMed等数据库以获取最新进展。
MAD3 (Mitotic Arrest Deficient 3) antibody is primarily associated with the detection of BubR1. a critical protein involved in the spindle assembly checkpoint (SAC) during mitosis. BubR1. encoded by the *BUB1B* gene, ensures accurate chromosome segregation by delaying anaphase until all chromosomes are properly attached to spindle microtubules. Dysfunction in BubR1 is linked to chromosomal instability, a hallmark of cancer, and has been implicated in aging-related disorders.
The MAD3 antibody recognizes BubR1. enabling researchers to study its expression, localization, and interactions in cell cycle regulation. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate SAC mechanisms, mitotic errors, and cellular responses to anti-mitotic drugs. Studies using MAD3 antibodies have revealed BubR1's role in aging, as reduced levels correlate with age-related phenotypes in model organisms. Additionally, its involvement in cancer progression makes it a potential biomarker or therapeutic target.
Originally identified in yeast, the term "MAD3" is sometimes used interchangeably with BubR1 in vertebrates, though nomenclature varies. Researchers employ MAD3 antibodies to explore molecular pathways governing genomic stability, providing insights into diseases like aneuploidy-driven cancers and developmental disorders. Its utility spans basic cell biology, translational oncology, and aging research.
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