| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G1P2; hUCRP; IFI15; IP17; ISG15; UCRP;;ISG15 |
| WB Predicted band size | Calculated MW: 18 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ISG15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ISG15抗体的3篇参考文献，按领域和内容分类简要概括：
1. **文献名称**：*ISG15 inhibits Nedd4 ubiquitin E3 activity and enhances the innate antiviral response*
**作者**：Malakhova OA et al.
**摘要**：该研究发现ISG15通过其抗体可特异性识别并结合E3泛素连接酶Nedd4.抑制其活性，从而增强干扰素信号通路，提高宿主细胞对病毒感染的先天免疫应答能力。
2. **文献名称**：*Antibody screening identifies anti-ISG15 antibodies as potent inhibitors of USP18-mediated deISGylation*
**作者**：Ketscher L et al.
**摘要**：研究团队利用单克隆抗体筛选技术，发现特定抗ISG15抗体可阻断去ISGylation酶USP18的功能，导致细胞内ISG15共轭蛋白积累，证实其在调控病毒感染和肿瘤免疫中的潜在应用价值。
3. **文献名称**：*Detection of ISG15 serological biomarkers in autoimmune diseases using a novel monoclonal antibody-based ELISA*
**作者**：Zhang X et al.
**摘要**：该研究开发了一种基于高特异性ISG15单克隆抗体的ELISA检测方法，用于系统性红斑狼疮(SLE)等自身免疫病患者血清中ISG15水平的定量分析，揭示其与疾病活动度的相关性。
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**领域关联性说明**：
以上文献覆盖ISG15抗体在**先天免疫调控**(病毒防御)、**酶活性干预**(药物开发)及**临床诊断**(生物标志物检测)三个核心研究方向，均涉及抗体在机制研究或转化医学中的关键应用。
**Background of ISG15 Antibodies**
ISG15 (Interferon-stimulated gene 15) is a ubiquitin-like protein induced by type I interferons (IFNs) as part of the innate immune response to viral infections, cellular stress, or inflammatory signals. It functions as a post-translational modifier through a process called ISGylation, where ISG15 conjugates to target proteins via sequential enzymatic steps analogous to ubiquitination. This modification regulates diverse cellular processes, including antiviral defense, protein stability, and immune signaling.
ISG15 antibodies are critical tools for studying its expression, localization, and interaction networks. They enable the detection of both free ISG15 and ISG15-conjugated proteins in techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Researchers use these antibodies to explore ISG15's role in viral pathogenesis (e.g., HIV, influenza, SARS-CoV-2), autoimmune disorders, and cancer, where dysregulated ISG15 expression is often observed.
Notably, ISG15 exists in two forms: a mature 15-kDa protein and a precursor with an additional C-terminal extension. Antibodies targeting specific epitopes or cleavage sites help distinguish these forms. Additionally, ISG15 antibodies aid in validating ISG15 knockout models and assessing its interplay with ubiquitin pathways. Their development has advanced understanding of ISG15's dual roles—acting as an immunomodulatory cytokine when secreted and as a post-translational modifier intracellularly. Such insights highlight ISG15's potential as a therapeutic target or biomarker in infectious and inflammatory diseases.
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