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产品描述：人干扰素诱导GTP结合蛋白Mx1(MX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆、细胞裂解物样本中的MX1含量。MX1 是一个值得关注的靶点。它在抗病毒、免疫调节等方面可能有重要作用。研究机制或许围绕其在细胞内信号传导途径中的功能展开，探索其如何响应外界刺激并调节细胞生理活动，有望为相关疾病的治疗提供新的思路和方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于病毒与宿主相互作用机制研究、干扰素信号通路分析以及抗病毒药物筛选等科研场景。为探索Mx1蛋白在天然免疫中的功能及其调控网络提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Human interferon regulated resistance GTP binding protein MXA ELISA Kit; IFI 78 ELISA Kit; IFI 78K ELISA Kit; IFI-78K ELISA Kit; IFI78 ELISA Kit; IFI78K ELISA Kit; Interferon induced GTP binding protein Mx1 ELISA Kit; Interferon induced protein p78 ELISA Kit; Interferon inducible protein p78 ELISA Kit; Interferon regulated resistance GTP binding protein ELISA Kit; Interferon regulated resistance GTP binding protein MxA ELISA Kit; Interferon-induced GTP-binding protein Mx1 ELISA Kit; Interferon-induced protein p78 ELISA Kit; Interferon-regulated resistance GTP-binding protein MxA ELISA Kit; MX 1 ELISA Kit; MX ELISA Kit; MX dynamin-like GTPase 1 ELISA Kit; MX1 ELISA Kit; MX1_HUMAN ELISA Kit; MxA ELISA Kit; Myxoma resistance protein 1 ELISA Kit; Myxovirus (influenza virus) resistance 1 interferon inducible ELISA Kit; Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 ELISA Kit; Myxovirus (influenza) resistance 1 homolog of murine ELISA Kit; Myxovirus resistance 1; mouse; homolog of ELISA Kit; Myxovirus resistance protein 1 ELISA Kit; N-terminally processed ELISA Kit
缩写：MX1
Uniprot No.：P20591
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 106 | ||||
| Range % | 98-110 | |||||
回收率：
| The recovery of human MX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.9181.8021.8601.7695001.2261.1561.1911.1002500.6940.6640.6790.5881250.4120.3980.4050.31462.50.2550.2500.2530.16231.250.1730.1690.1710.08015.60.1230.1220.1230.03200.0910.0900.091 | ||||||
货期：3-5 working days
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