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产品描述：人粘蛋白/粘液素7(MUC7)酶联免疫试剂盒检测血清、血浆、组织培养上清液、细胞裂解物,唾液样本中的MUC7含量。MUC7是一种重要靶点。它是一种粘蛋白，在人体呼吸道、口腔等黏膜表面发挥保护作用。研究机制方面，围绕其结构与功能展开，探索其在疾病发生发展中的角色，如在呼吸道感染、口腔疾病中，MUC7可能通过影响病原体黏附等机制参与其中。试剂盒检测范围为18.75 ng/mL-1200 ng/mL，适用于科研领域探究口腔疾病、呼吸道炎症、微生物互作等方向中粘蛋白的动态变化，为黏膜免疫机制研究、唾液成分分析及体外细胞模型分泌功能评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apo MG2 ELISA Kit; Apo-MG2 ELISA Kit; DKFZp686J03256 ELISA Kit; FLJ27047 ELISA Kit; MG2 ELISA Kit; MGC34772 ELISA Kit; MUC 7 ELISA Kit; MUC-7 ELISA Kit; MUC7 ELISA Kit; MUC7_HUMAN ELISA Kit; Mucin 7 ELISA Kit; Mucin 7; salivary ELISA Kit; Mucin 7; secreted ELISA Kit; Mucin-7 ELISA Kit; Salivary mucin 7 ELISA Kit; Salivary mucin-7 ELISA Kit
缩写：MUC7
Uniprot No.：Q8TAX7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates,saliva
检测范围：18.75 ng/mL-1200 ng/mL
灵敏度：4.68 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 89 | |
| Range % | 85-105 | ||
| 1:2 | Average % | 95 | |
| Range % | 90-100 | ||
| 1:4 | Average % | 99 | |
| Range % | 92-107 | ||
| 1:8 | Average % | 92 | |
| Range % | 86-98 |
回收率：
| The recovery of human MUC7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-103 | ||||
| EDTA plasma (n=4) | 91 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 12002.0521.9522.0021.902 6001.3251.3761.3511.251 3000.8760.8450.8610.761 1500.5290.5250.5270.427 750.3480.3570.3530.253 37.50.2630.2740.2690.169 18.750.1850.1740.1800.080 00.1020.0970.100 | ||||||
货期：3-5 working days
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