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产品描述：人骨骼肌特异性受体酪氨酸激酶(MUSK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MUSK含量。MUSK作为一种靶点，其具体背景可能涉及在特定生理过程或疾病中发挥关键作用。研究机制方面，可能是通过与特定配体结合参与信号传导通路，对细胞的生长、分化等过程产生影响，目前相关研究或致力于基于此靶点开发针对性的治疗药物或干预手段。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于基础科研中探索肌肉发育、再生机制或神经肌肉相关疾病的分子机理研究。科研人员可通过该产品分析不同生理或病理条件下MUSK的动态变化，为神经肌肉调控、药物筛选等研究提供可靠数据支持，但不可用于临床诊断。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MUSK; Muscle, skeletal receptor tyrosine-protein kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor
缩写：MUSK
Uniprot No.：O15146
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUSK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 99-109 | |||||
回收率：
| The recovery of human MUSK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-99 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.6752.5522.6142.510502.2262.3762.3012.197251.6721.5941.6331.52912.50.7920.8160.8040.7006.250.4460.4270.4370.3333.120.2360.2310.2340.1301.560.1800.1890.1850.08100.1060.1020.104 | ||||||
货期：3-5 working days
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