| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BOB 1; OBF1; OCAB; OCT binding factor 1; Pou2af1;;POU2AF1 |
| WB Predicted band size | Calculated MW: 27 kDa ; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human POU2AF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于BOB1抗体的3篇代表性文献，按作者和内容概括整理：
---
1. **文献名称**：*BOB.1. OCT2. and PU.1 in Hodgkin lymphoma: Association with clinicopathologic features and comparative utility*
**作者**：Hartmann S et al.
**摘要**：该研究评估BOB1抗体在霍奇金淋巴瘤(HL)中的诊断价值，发现BOB1与OCT2联合表达可帮助区分经典型HL的亚型，并提示其与患者预后的潜在关联。
2. **文献名称**：*Utility of BOB.1 and OCT2 antibodies in the classification of malignant lymphomas*
**作者**：Krenács L et al.
**摘要**：研究证实BOB1抗体在鉴别B细胞非霍奇金淋巴瘤中的特异性，强调其与OCT2共表达在区分生发中心与非生发中心来源肿瘤中的关键作用。
3. **文献名称**：*The role of BOB.1 in B-cell development and lymphomagenesis*
**作者**：Schneider M et al.
**摘要**：探讨BOB1在B细胞分化中的分子机制，通过抗体标记揭示其在淋巴瘤中的异常表达模式，为诊断及靶向治疗提供理论依据。
---
**注**：BOB1(又名OBF1或OCA-B)抗体常用于病理诊断，尤其在区分霍奇金淋巴瘤亚型及B细胞肿瘤分类中具有重要价值。以上文献均从不同角度验证其临床应用。如需具体年份或DOI，可进一步补充说明。
BOB1 (also known as OBF1 or OCA-B) is a B-cell-specific transcriptional coactivator encoded by the *POU2AF1* gene. It plays a critical role in B-cell development and immune response by interacting with transcription factors OCT1/OCT2 (POU2F1/POU2F2) to regulate the expression of immunoglobulin genes and other B-cell-specific targets. BOB1 stabilizes OCT1/2 binding to DNA, enhancing transcriptional activation of genes involved in antibody production, B-cell receptor signaling, and antigen presentation.
BOB1 antibodies are widely used in research and diagnostic pathology to identify B-cell lineage in lymphoid malignancies. In diagnostic immunohistochemistry, BOB1 is often co-expressed with OCT2 in classical Hodgkin lymphoma (cHL) and certain non-Hodgkin lymphomas (e.g., nodular lymphocyte-predominant HL), aiding in subtype classification. Its absence or reduced expression may correlate with immunodeficiency disorders or aberrant B-cell differentiation.
As a marker, BOB1 helps distinguish B-cell neoplasms from T-cell or myeloid malignancies. Its utility is enhanced when combined with OCT2. as dual expression supports cHL diagnosis. Studies also explore its role in autoimmune diseases and immune checkpoint regulation. Commercial BOB1 antibodies target specific epitopes, validated for applications in Western blot, flow cytometry, and tissue staining, contributing to both basic research and clinical diagnostics.
×
