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产品描述：人粘蛋白/粘液素5B(MUC5B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MUC5B含量。MUC5B是一种与疾病相关的靶点。它在呼吸道黏液分泌中扮演重要角色。研究发现其异常表达可能引发呼吸道疾病如慢性阻塞性肺疾病等。机制上，主要聚焦于其基因表达调控、蛋白功能及与其他细胞因子相互作用等方面，以探索潜在治疗干预点。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于基础医学研究、疾病机制探索或药物开发中 MUC5B 表达水平的动态监测，例如评估肺纤维化模型动物黏液分泌状态、分析炎症性肠病患者组织样本的黏液层变化等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2300002I04Rik ELISA Kit; A130042M24 ELISA Kit; AV085033 ELISA Kit; Cervical mucin ELISA Kit; Cervical mucin MUC5B ELISA Kit; High molecular weight salivary mucin MG1 ELISA Kit; MG1 ELISA Kit; MUC-5B ELISA Kit; MUC5 ELISA Kit; MUC5B ELISA Kit; MUC5B_HUMAN ELISA Kit; MUC9 ELISA Kit; Mucin 5 subtype B, tracheobronchial ELISA Kit; Mucin 5B ELISA Kit; Mucin 5B oligomeric mucus/gel forming ELISA Kit; Mucin-5 subtype B ELISA Kit; Mucin-5B ELISA Kit; Sublingual gland mucin ELISA Kit; tracheobronchial ELISA Kit
缩写：MUC5B
Uniprot No.：Q9HC84
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-108 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.5082.6072.5582.4152501.7651.8641.8151.6721251.1871.2061.1971.05462.50.7910.8010.7960.65331.20.4470.4380.4430.30015.60.3020.3110.3070.1647.80.2380.2320.2350.09200.1420.1430.143 | ||||||
货期：3-5 working days
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