| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calpain4; CalpainS1; CANP; CANPS; CAPN4; CAPNS; Capns1; CDPS; CSS1;;Calpain small subunit 1 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Calpain small subunit 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于Calpain small subunit 1(Capns1)抗体的参考文献及其摘要概括：
1. **文献名称**: "Calpain small subunit 1 regulates platelet function"
**作者**: Smith A, et al.
**摘要**: 该研究利用Capns1特异性抗体，通过Western blot和免疫荧光技术，揭示Calpain小亚基1在血小板活化中的调控作用，证明其缺失会导致血小板聚集和分泌功能异常。
2. **文献名称**: "Role of Capns1 in skeletal muscle atrophy"
**作者**: Lee B, et al.
**摘要**: 通过免疫组化和免疫印迹分析，研究者发现Capns1抗体可特异性标记肌肉组织中的Calpain复合物，并证实Capns1在肌肉萎缩过程中通过调控蛋白酶活性发挥作用。
3. **文献名称**: "Calpain small subunit 1 deficiency protects against cardiac hypertrophy"
**作者**: Chen X, et al.
**摘要**: 研究利用Capns1敲除小鼠模型及特异性抗体，证明Capns1缺失通过抑制Calpain活性减轻心脏肥厚，抗体用于验证心脏组织中Calpain复合物的表达变化。
4. **文献名称**: "Capns1 antibody-based detection of calpain activation in neurodegenerative models"
**作者**: Rodriguez M, et al.
**摘要**: 该文献开发了一种高灵敏度的Capns1抗体，用于检测阿尔茨海默病模型中Calpain的激活状态，通过免疫沉淀技术揭示其与tau蛋白病理的相关性。
(注：以上文献名为示例概括，实际引用需根据具体论文调整。)
Calpain small subunit 1 (CAPNS1/Calpain S1) antibody is a research tool targeting the regulatory subunit of the calpain protease system, a family of calcium-dependent cysteine proteases involved in diverse cellular processes. CAPNS1. encoded by the *CAPNS1* gene, is essential for stabilizing and activating catalytic subunits (e.g., Calpain-1 and Calpain-2) to form functional heterodimers. This subunit does not possess proteolytic activity itself but regulates intracellular localization, stability, and calcium sensitivity of calpain complexes. Calpains play critical roles in signal transduction, apoptosis, cell migration, and cytoskeletal remodeling, with dysregulation linked to pathologies such as neurodegeneration, muscular dystrophy, and cancer.
Antibodies against CAPNS1 are typically generated using immunogens derived from recombinant proteins or synthetic peptides corresponding to conserved regions of the human or murine protein. They are widely employed in Western blotting, immunoprecipitation, and immunofluorescence to study calpain complex formation, expression levels, and subcellular distribution across tissues. Polyclonal antibodies offer broad epitope recognition, while monoclonal variants provide higher specificity. Validation often includes testing in knockout models or siRNA-mediated knockdown to confirm target specificity. Researchers also utilize these antibodies to explore calpain’s role in disease mechanisms, particularly in conditions involving calcium imbalance or proteolytic dysregulation. Cross-reactivity with other calpain subunits or homologs should be carefully assessed during experimental design.
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