首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人巨噬细胞清道夫受体I和II(MSR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MSR1含量。MSR1即巨噬细胞清道夫受体 1，广泛表达于巨噬细胞表面，参与清除体内修饰脂蛋白及病原体等。研究机制上，其与动脉粥样硬化、肿瘤等疾病密切相关，能通过介导巨噬细胞摄取脂质促进斑块形成，也可影响肿瘤微环境免疫状态。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于心血管疾病机制研究、巨噬细胞功能探索、炎症信号通路分析等科研领域，为体外实验提供稳定可靠的蛋白浓度数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MSR1; SCARA1; Macrophage scavenger receptor types I and II; Macrophage acetylated LDL receptor I and II; Scavenger receptor class A member 1; CD antigen CD204
缩写：MSR1
Uniprot No.：P21757
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of human MSR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.3422.1642.2532.11925001.6761.7721.7241.59012501.1941.1531.1741.0406250.7640.7510.7580.6243120.4520.4490.4510.3171560.3130.3010.3070.173780.2220.2140.2180.08400.1340.1330.134 | ||||||
货期：3-5 working days
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