关键信息
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基因名
MYCN
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简介
The MYCN protein is critical in neuroblastoma cells and can positively regulate MYCNOS transcription through DNA binding, requiring heterodimerization with MAX. Interactions with KDM5A, KDM5B, and HUWE1 indicate involvement in multiple cellular processes. MYCN Protein, Human (Sf9, His) is the recombinant human-derived MYCN, expressed by Sf9 insect cells, with C-6*His labeled tag.
- 应用
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别名
bHLHe37; Class E basic helix-loop-helix protein 37; MODED; mycn; Oncogene NMYC; pp65/67
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种属
Human
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表达系统
Baculovirus
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04198
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表达区间
M1-C464
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氨基酸序列
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC
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蛋白长度
Full Length
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MYCN is a member of the MYC family of oncogenes and is known to play a critical role in the development and progression of neuroblastoma, a common pediatric cancer. Amplification of the MYCN gene is associated with aggressive tumor behavior and poor prognosis in affected children. Research into MYCN has focused on understanding its biological functions, regulatory mechanisms, and interactions with other signaling pathways in oncogenesis. The production of recombinant MYCN proteins has become increasingly important for elucidating the molecular mechanisms underlying its activity and for identifying potential therapeutic targets. By using techniques such as recombinant DNA technology and protein expression systems, researchers can produce MYCN in a controlled laboratory setting, allowing detailed studies of its structure, function, and role in cell signaling. Such investigations have the potential to reveal novel inhibitors that could disrupt MYCN-driven tumor growth, ultimately leading to the development of more effective therapies for neuroblastoma and improving outcomes for patients with MYCN-amplified tumors. This area of research thus holds significant promise for advancing our understanding of cancer biology and for guiding the search for new treatment strategies.
