| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;DiMethyl-Histone H3 (K5) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of K5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Histone H3(dimethylK4)抗体的3篇参考文献，简要概括如下：
1. **文献名称**：*"Selective recognition of methylated lysine 4 on histone H3 by the HP1 chromo domain"*
**作者**：Bannister, A.J., et al.
**摘要**：该研究鉴定了组蛋白H3K4甲基化(包括二甲基化)与染色质结合蛋白HP1的相互作用，揭示了H3K4me2在异染色质形成中的潜在作用，并验证了抗体在染色质免疫沉淀(ChIP)中的特异性。
2. **文献名称**：*"Active genes are tri-methylated at K4 of histone H3"*
**作者**：Santos-Rosa, H., et al.
**摘要**：通过使用H3K4me2特异性抗体，研究发现H3K4二甲基化在酵母基因启动子区富集，且与转录活性相关，为表观遗传调控基因表达的机制提供了证据。
3. **文献名称**：*"Methylation of histone H3K4 mediates association of the methylation machinery with active genes"*
**作者**：Bernstein, B.E., et al.
**摘要**：该文献利用H3K4me2抗体分析胚胎干细胞分化过程中的组蛋白修饰动态，发现H3K4me2标记在多能性基因启动子区的维持对细胞命运决定至关重要。
4. **文献名称**：*"Antibody-specific detection of dimethylated histone H3K4 in embryonic stem cells"*
**作者**：Cheng, X., & Blum, R.
**摘要**：研究优化了H3K4me2抗体的应用条件，验证其在胚胎干细胞中的特异性，并揭示了该修饰在维持干细胞自我更新中的功能。
以上文献均涉及H3K4me2抗体的应用及其在基因调控和表观遗传机制中的研究。
The Histone H3 (dimethyl K4) antibody is a key tool for studying epigenetic regulation, specifically targeting the dimethylation of lysine 4 on histone H3 (H3K4me2). This post-translational modification is enriched in promoter regions of actively transcribed genes and is associated with transcriptional activation. H3K4me2 is deposited by methyltransferases such as SET1/COMPASS complexes and removed by demethylases like LSD1/KDM1A or JARID1/KDM5 family proteins. It plays a critical role in chromatin remodeling, maintaining an open chromatin state to facilitate transcription factor binding and RNA polymerase II recruitment.
Researchers use this antibody in techniques like chromatin immunoprecipitation (ChIP-seq), immunofluorescence (IF), and Western blotting (WB) to map H3K4me2 distribution and investigate its involvement in cellular processes, including differentiation, development, and disease. Aberrant H3K4me2 levels have been linked to cancers, neurological disorders, and metabolic syndromes, making the antibody valuable for both basic research and clinical studies.
Specificity validation is essential, as cross-reactivity with mono- or trimethylated H3K4 or other histone modifications can occur. Proper controls (e.g., knockout cells or peptide competition assays) are required to confirm antibody reliability. Commercial antibodies often provide validation data across multiple applications, but batch variability may affect performance. Understanding H3K4me2 dynamics through this antibody aids in deciphering gene regulatory mechanisms and identifying therapeutic targets in epigenetic diseases.
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