首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠半乳糖凝集素-3(LGALS3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LGALS3含量。LGALS3 是一种具有重要生物学功能的靶点。它参与细胞黏附、增殖、凋亡等多种生理过程。在研究机制上，其与肿瘤发生、发展和免疫逃逸密切相关，还涉及纤维化等疾病进程。对它的深入研究有助于开发新的疾病诊断方法和治疗策略。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于探索大鼠模型中纤维化疾病进展、肿瘤发生机制、代谢综合征相关病理变化或免疫调节研究，为动物实验提供可靠的蛋白定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
缩写：LGALS3
Uniprot No.：P08699
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LGALS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of rat LGALS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 87 | 83-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.7712.7982.7852.621502.0662.0982.0821.918251.4121.4001.4061.24212.50.8410.8680.8550.6916.250.5970.5750.5860.4223.120.4660.4780.4720.3081.560.3180.3360.3270.16300.1630.1650.164 | ||||||
货期：3-5 working days
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