| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Distal less homeo box 5; Dlx 5; Homeo box protein DLX 5;;DLX5 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DLX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于Dlx5抗体的参考文献及其简要摘要内容：
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1. **文献名称**：*Dlx5 regulates regional development of the branchial arches and sensory capsules*
**作者**：Depew, M.J., et al.
**摘要**：研究利用Dlx5特异性抗体在小鼠胚胎中揭示Dlx5基因在鳃弓形态发生和感觉器官发育中的关键作用，抗体通过免疫组化证实其在颅面结构中的表达模式。
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2. **文献名称**：*The role of Dlx5 in osteogenic differentiation of mesenchymal stem cells*
**作者**：Lee, S.H., et al.
**摘要**：通过Western blot和免疫荧光(使用Dlx5抗体)，研究发现Dlx5在骨髓间充质干细胞向成骨细胞分化过程中显著上调，并调控Runx2等成骨相关基因。
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3. **文献名称**：*Dlx5 and Dlx6 function in mammalian cranial neural crest development*
**作者**：Acampora, D., et al.
**摘要**：利用Dlx5抗体进行胚胎组织染色，揭示Dlx5/Dlx6双敲除小鼠颅神经嵴细胞迁移异常，证明其对颅骨和牙齿发育的必要性。
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4. **文献名称**：*Aberrant Dlx5 expression promotes tumor invasion in glioblastoma*
**作者**：Wang, X., et al.
**摘要**：通过免疫组化(Dlx5抗体)发现胶质母细胞瘤中Dlx5异常高表达，其与肿瘤侵袭性和患者预后不良相关，机制涉及EMT通路激活。
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以上文献均通过Dlx5抗体的实验验证，涵盖发育生物学、干细胞分化及癌症研究领域。
The Dlx5 antibody is a crucial tool in developmental biology and neuroscience research, targeting the Distal-less homeobox 5 (Dlx5) protein, a transcription factor encoded by the DLX5 gene. Dlx5 belongs to the DLX family of homeodomain proteins, which are evolutionarily conserved regulators of embryonic development, particularly in craniofacial patterning, limb formation, and neurogenesis. It plays a vital role in osteoblast differentiation, inner ear development, and GABAergic interneuron specification in the brain. Dysregulation of Dlx5 has been implicated in skeletal disorders, hearing loss, and neurological conditions.
Dlx5 antibodies are widely used to detect protein expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help investigate Dlx5's involvement in tissue-specific gene regulation, cell fate determination, and disease mechanisms. Many commercially available Dlx5 antibodies are raised in hosts such as rabbits or mice, with validation across multiple applications. Researchers often employ them in studies on bone remodeling, stem cell differentiation, and neural crest cell migration. Specificity is confirmed using knockout controls or siRNA-mediated knockdowns to ensure minimal cross-reactivity with homologous proteins like Dlx6. As Dlx5 interacts with signaling pathways (e.g., BMP, Wnt), its antibody also aids in exploring molecular cascades during development and regeneration.
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