| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADH; ADH1; ADH1A; Aldehyde reductase;;ADH1A |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ADH1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Alcohol Dehydrogenase(ADH)抗体的3篇参考文献示例(内容为虚构模拟，仅供参考)：
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1. **文献名称**: *"Production and Characterization of a Monoclonal Antibody Against Human Liver Alcohol Dehydrogenase"*
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种针对人肝脏ADH的单克隆抗体，通过免疫印迹和免疫组化验证其特异性。该抗体能够识别ADH1B亚型，适用于定量分析肝脏组织中ADH的表达水平，为酒精代谢相关疾病的研究提供工具。
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2. **文献名称**: *"Immunohistochemical Localization of Alcohol Dehydrogenase in Mouse Tissues Using a Polyclonal Antibody"*
**作者**: Lee H, et al.
**摘要**: 利用兔源多克隆抗体，通过免疫组织化学技术定位ADH在小鼠肝脏、胃和脑组织中的分布。研究发现ADH在胃黏膜中的高表达可能与局部乙醇代谢有关，提示其在胃肠道保护中的作用。
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3. **文献名称**: *"A Novel Antibody-Based Assay for Detecting Alcohol Dehydrogenase Activity in Plant Extracts"*
**作者**: Garcia R, et al.
**摘要**: 开发了一种基于抗ADH抗体的ELISA方法，用于检测植物(如葡萄和苹果)提取物中的ADH酶活性。该方法灵敏度高，可应用于植物抗逆性及果实成熟度的生化研究。
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**备注**：以上文献为示例，实际引用需根据具体研究需求检索PubMed、Web of Science等数据库获取真实文献(如搜索关键词：Alcohol Dehydrogenase antibody, ADH immunoassay)。
Alcohol dehydrogenase (ADH) antibodies are immunological tools designed to detect and study ADH enzymes, a family of NAD⁺-dependent enzymes critical in metabolizing ethanol, retinols, and other alcohols. ADH exists in multiple isoforms (e.g., ADH1A, ADH1B, ADH1C) encoded by distinct genes, with tissue-specific expression patterns. Class I ADH isoforms (ADH1A-C) are primarily expressed in the liver and gastrointestinal tract, playing a central role in ethanol oxidation, while other classes participate in retinoid metabolism or steroid homeostasis.
ADH antibodies, typically raised in rabbits, mice, or goats, are generated using purified ADH proteins or synthetic peptides as immunogens. These antibodies enable researchers to quantify ADH expression via techniques like Western blotting, immunohistochemistry (IHC), or ELISA, and to study its localization in tissues. Their applications span alcohol metabolism research, liver disease studies (e.g., cirrhosis, hepatitis), and investigations into cancer progression, as altered ADH expression is linked to tumorigenesis in certain cancers.
Specificity validation is critical, as ADH isoforms share structural homology. High-quality antibodies distinguish between isoforms, aiding research on genetic polymorphisms (e.g., ADH1B*2 variant associated with reduced alcohol dependence risk). Additionally, ADH antibodies are used in toxicology to assess ethanol-induced organ damage and in pharmacogenomics to explore interindividual variability in drug/alcohol responses. Proper controls, including knockout tissues or competitive peptides, ensure antibody reliability in experimental models.
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