首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠低密度脂蛋白受体(LDLR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDLR含量。LDLR（低密度脂蛋白受体）是一种在细胞膜上存在的蛋白质，主要功能是降解血液中的低密度脂蛋白（LDL），从而维持胆固醇的稳态。LDLR的表达和功能受到多种因素的调节，包括胆固醇水平、胰岛素、甲状腺激素等。LDLR缺陷或功能障碍与多种疾病，如家族性高胆固醇血症和动脉粥样硬化有关。研究LDLR的机制有助于开发新的治疗策略来预防和治疗这些疾病。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于基础科研中探究高胆固醇饮食模型、心血管疾病动物模型的LDLR动态变化，以及药物干预对脂质代谢通路影响的机制研究。适用于肝脏、血管等组织样本与体液样本的平行检测，为脂质代谢相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ldlr ELISA Kit; Low-density lipoprotein receptor ELISA Kit; LDL receptor ELISA Kit
缩写：LDLR
Uniprot No.：P35952
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:200 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:400 | Average % | 102 | |||||
| Range % | 98-108 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:1600 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of rat LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 93 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.5122.4752.4942.3552502.2472.1962.2222.0831251.8031.6891.7461.60762.51.1891.2271.2081.06931.20.6870.7130.7000.56115.60.4150.3920.4040.2657.80.2980.2890.2940.15500.1360.1410.139 | |||||||
货期：3-5 working days
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