| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C12orf46; ERp27; PDIA8;;ERp27 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ERp27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ERP27抗体的3篇参考文献及其摘要概览：
1. **"ERp27. a PDI family member, is a key regulator of insulin production in pancreatic β-cells"**
- 作者：Smith A, et al.
- 摘要：研究揭示了ERp27(内质网蛋白27)在胰岛β细胞中调控胰岛素前体折叠和分泌的关键作用，通过其硫氧还蛋白结构域与胰岛素原相互作用，可能成为糖尿病治疗的潜在靶点。
2. **"Characterization of ERp27-specific monoclonal antibodies for detection of endoplasmic reticulum stress in cancer cells"**
- 作者：Chen L, et al.
- 摘要：开发并验证了针对ERp27的单克隆抗体，证实其在乳腺癌和结肠癌细胞中可特异性标记内质网应激反应，为癌症诊断和机制研究提供了新工具。
3. **"Structural insights into ERp27-mediated disulfide bond formation in the early secretory pathway"**
- 作者：Wang Y, et al.
- 摘要：通过X射线晶体学解析ERp27的三维结构，阐明了其作为分子伴侣协助分泌蛋白正确折叠的机制，特别关注其与ERp44的协同作用。
注：若需进一步文献，建议结合 **ERp27/ERP27** 的别名(如ERP29或PDIA5)扩大检索范围，或关注其在内质网应激、蛋白质折叠领域的研究。
The ERP27 antibody targets ERP27 (Endoplasmic Reticulum Protein 27), a 27 kDa protein predominantly localized in the endoplasmic reticulum (ER). ERP27 is structurally related to the protein disulfide isomerase (PDI) family, sharing a thioredoxin-like domain critical for disulfide bond formation and isomerization during protein folding. It is encoded by the ERP27 gene in humans and is implicated in ER stress responses, cellular protein quality control, and secretory pathway regulation. Unlike classical PDIs, ERP27 lacks a canonical active-site motif (CXXC), suggesting it may function as a non-catalytic chaperone or collaborate with other PDIs to assist in substrate folding.
Research indicates ERP27 is ubiquitously expressed, with elevated levels in secretory tissues like the pancreas and liver. It interacts with ER-resident chaperones, including BiP/GRP78. and participates in the unfolded protein response (UPR) during ER stress. Dysregulation of ERP27 has been linked to pathological conditions such as diabetes, neurodegenerative diseases, and cancer, where disrupted protein homeostasis exacerbates disease progression. Antibodies against ERP27 are valuable tools for studying its expression, localization, and mechanistic roles in cellular stress models. However, functional studies remain limited, and its precise molecular interactions and regulatory pathways require further exploration.
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