首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人乳酸脱氢酶A (LDHA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LDHA含量。LDHA即乳酸脱氢酶A，在糖酵解代谢通路中发挥关键作用，可催化丙酮酸和乳酸的可逆转换。其表达异常与肿瘤细胞的能量代谢重编程密切相关，是肿瘤治疗的重要潜在靶点，研究聚焦于开发其抑制剂以阻断肿瘤代谢，抑制肿瘤生长。试剂盒检测范围为31.25 mU/mL-2000 mU/mL，适用于体外培养细胞能量代谢评估、动物模型代谢动态分析、抗肿瘤药物筛选等科研场景，特别为研究肿瘤瓦伯格效应、缺氧应激反应或代谢相关信号通路提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell proliferation-inducing gene 19 protein ELISA Kit; GSD11 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; Lactate dehydrogenase A ELISA Kit; Lactate dehydrogenase B ELISA Kit; Lactate dehydrogenase c variant 1 ELISA Kit; Lactate dehydrogenase c variant 3 ELISA Kit; Lactate dehydrogenase c variant 4 ELISA Kit; Lactate dehydrogenase C4 ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; Lactate dehydrogenase M ELISA Kit; LDH A ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH M ELISA Kit; LDH muscle subunit ELISA Kit; LDH-A ELISA Kit; LDH-M ELISA Kit; LDH1 ELISA Kit; ldha ELISA Kit; LDHA_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHM ELISA Kit; MS1111 ELISA Kit; PIG19 ELISA Kit; Proliferation inducing gene 19 ELISA Kit; Proliferation-inducing gene 19 ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-59 ELISA Kit; TRG 5 ELISA Kit; TRG5 ELISA Kit
缩写：LDHA
Uniprot No.：P00338
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 mU/mL-2000 mU/mL
灵敏度：7.81 mU/Ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LDHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 88 | |||||||||||
| Range % | 81-92 | ||||||||||||
| 1:2 | Average % | 99 | |||||||||||
| Range % | 94-102 | ||||||||||||
| 1:4 | Average % | 101 | |||||||||||
| Range % | 97-105 | ||||||||||||
| 1:8 | Average % | 94 | |||||||||||
| Range % | 88-98 | ||||||||||||
回收率：
| The recovery of human LDHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 93 | 89-96 | |||||||||||
| EDTA plasma (n=4) | 96 | 94-98 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| mU/mlOD1OD2AverageCorrected20002.1011.9522.0271.91410001.6761.7341.7051.5925001.2521.2381.2451.1322500.7680.7580.7630.6501250.5660.5680.5670.45462.50.3280.3210.3250.21231.250.1790.1850.1820.06900.1140.1120.113 | |||||||||||||
货期：3-5 working days
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